Inducible gankyrin overexpression drives hepatocarcinogenesis in a liver-specific zebrafish model
文献类型：期刊论文
| 作者 | Gong, Zhiyuan5,6; Sun, Yuxi1,5; Lin, Yueh-Min2,3; Lu, Jeng-Wei4,5 |
| 刊名 | ACTA HISTOCHEMICA
 |
| 出版日期 | 2025-09-01 |
| 卷号 | 127期号:3页码:8 |
| 关键词 | Doxycycline Gankyrin Hyperplasia Hepatocellular carcinoma Zebrafish |
| ISSN号 | 0065-1281 |
| DOI | 10.1016/j.acthis.2025.152280 |
| 通讯作者 | Lu, Jeng-Wei(lujengwei@mail.ntou.edu.tw) |
| 英文摘要 | Background: Hepatocarcinogenesis is a complex, multistep process that begins with fatty liver, progresses to fibrosis, and ultimately leads to cancer. Numerous etiological factors contribute to this progression, highlighting the importance of developing animal models to facilitate both basic and translational research aimed at discovering new therapeutic strategies. Gankyrin is a key oncoprotein involved in the genetic regulation of liver pathology. Material and method: To investigate its oncogenic role without the need for cancer cell inoculation or drug treatment, we employed a Tet-On system to drive zebrafish gankyrin overexpression in hepatocytes under the control of the fabp10a promoter. Results: After eight weeks of induction, fabp10a:eGFP-gankyrin transgenic zebrafish spontaneously developed persistent hyperplasia, bile duct hyperplasia, and hepatocellular carcinoma (HCC), demonstrating the oncogenic potential of gankyrin in liver tumorigenesis. In this study, we demonstrate that gankyrin activation drives the progressive development of HCC in zebrafish. Liver-specific overexpression of gankyrin in wild-type zebrafish led to hyperplasia, bile duct hyperplasia, and HCC, establishing a robust zebrafish model for studying liver cancer. Our findings highlight the utility of this model for investigating the molecular mechanisms underlying tumorigenesis. Conclusion: This study establishes a robust zebrafish model in which liver-specific overexpression of gankyrin induces spontaneous progression from hyperplasia to hepatocellular carcinoma. The model provides a valuable platform for investigating the molecular mechanisms of hepatocarcinogenesis and exploring potential therapeutic strategies. |
| WOS关键词 | ONCOPROTEIN GANKYRIN ; HEPATOCELLULAR-CARCINOMA ; IN-VIVO ; EXPOSURE ROUTES ; GENE-EXPRESSION ; CANCER ; P53 ; PROTEIN ; TUMORIGENESIS ; DEGRADATION |
| 资助项目 | Ministry of Education, Singapore[R154000B88112] ; Ministry of Education, Singapore[R154000B70114] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001544836600001 |
| 出版者 | ELSEVIER GMBH |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202761]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Lu, Jeng-Wei |
| 作者单位 | 1.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China 2.Changhua Christian Hosp, Dept Pathol, Changhua, Taiwan 3.Chung Shan Med Univ, Sch Med, Taichung, Taiwan 4.Natl Taiwan Ocean Univ, Dept Biosci & Biotechnol, 2 Beining Rd, Keelung, Taiwan 5.Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore 6.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Zhiyuan,Sun, Yuxi,Lin, Yueh-Min,et al. Inducible gankyrin overexpression drives hepatocarcinogenesis in a liver-specific zebrafish model[J]. ACTA HISTOCHEMICA,2025,127(3):8. |
| APA | Gong, Zhiyuan,Sun, Yuxi,Lin, Yueh-Min,&Lu, Jeng-Wei.(2025).Inducible gankyrin overexpression drives hepatocarcinogenesis in a liver-specific zebrafish model.ACTA HISTOCHEMICA,127(3),8. |
| MLA | Gong, Zhiyuan,et al."Inducible gankyrin overexpression drives hepatocarcinogenesis in a liver-specific zebrafish model".ACTA HISTOCHEMICA 127.3(2025):8. |
入库方式： OAI收割
来源：海洋研究所
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