Repressing epigenetic regulation enhances exogenous gene expression in the macroalga Pyropia yezoensis (Rhodophyta)
文献类型：期刊论文
| 作者 | Wang, Hong1,2,3; Gu, Wenhui2,3; Xie, Xiujun2,3; Wang, Guangce2,3 |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2025-09-01 |
| 卷号 | 199期号:1页码:12 |
| ISSN号 | 0032-0889 |
| DOI | 10.1093/plphys/kiaf393 |
| 通讯作者 | Xie, Xiujun(xjxie@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Pyropia yezoensis, an economically valuable macroalga, occupies a pivotal position in evolutionary history as a red alga, making it an ideal model organism for investigating the evolution of photosynthesis. However, efficient genetic manipulation in P. yezoensis, particularly the stable expression of exogenous genes, presents substantial challenges. Major obstacles, including gene silencing, co-suppression, and unstable transgenerational inheritance, hinder progress in this field. Emerging evidence suggests that epigenetic modifications, including histone acetylation and deacetylation, play crucial roles in regulating endogenous gene expression. Nevertheless, whether these processes influence the expression of exogenous genes in P. yezoensis remains largely unexplored. In this study, we demonstrate that the expression of an exogenous gene can be enhanced by silencing the gene encoding Sin3-associated protein 30 (PySAP30), a critical component of the histone deacetylase (HDAC) complex. To evaluate exogenous gene expression in P. yezoensis, we employed the GUS reporter gene driven by the strong endogenous polyubiquitin gene promoter (PypUbi1). We designed a dual-ribozyme system, comprising hammerhead and hepatitis delta virus ribozymes, to generate antisense fragments targeting PySAP30 exons. This strategy effectively suppressed PySAP30 expression, resulting in a marked increase in both GUS transcript abundance and enzymatic activity. In contrast, transformants without PySAP30 interference showed low GUS activity and pronounced chimerism. Our findings confirm the repressive effect of epigenetic modification on exogenous gene expression in P. yezoensis and highlight a potential strategy for improving exogenous gene expression in macroalgae. Epigenetic regulation strategies pave the way for efficient exogenous gene expression, genetic improvement, and synthetic biology in macroalgae. |
| WOS关键词 | HISTONE ACETYLATION ; RED ; METABOLISM ; EVOLUTION ; MARKER ; PLANT |
| 资助项目 | National Natural Science Foundation of China[41876160] ; Key Technology Research Projects in Qingdao City[25-1-1-gjgg-54-hy] ; Key R&D Program of Shandong Province[2025CXGC010618] ; Shandong Provincial Key Research and Development Program[2022LZGC004] ; Natural Science Foundation of Qingdao ; Foundation of State Key Laboratory of Mariculture Biobreeding ; Sustainable Goods[BRESG202308] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; China Agriculture Research System of MOF ; Major Scientific and Technological Innovation Project |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001574525200001 |
| 出版者 | OXFORD UNIV PRESS INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203431]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Xie, Xiujun; Wang, Guangce |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hong,Gu, Wenhui,Xie, Xiujun,et al. Repressing epigenetic regulation enhances exogenous gene expression in the macroalga Pyropia yezoensis (Rhodophyta)[J]. PLANT PHYSIOLOGY,2025,199(1):12. |
| APA | Wang, Hong,Gu, Wenhui,Xie, Xiujun,&Wang, Guangce.(2025).Repressing epigenetic regulation enhances exogenous gene expression in the macroalga Pyropia yezoensis (Rhodophyta).PLANT PHYSIOLOGY,199(1),12. |
| MLA | Wang, Hong,et al."Repressing epigenetic regulation enhances exogenous gene expression in the macroalga Pyropia yezoensis (Rhodophyta)".PLANT PHYSIOLOGY 199.1(2025):12. |
入库方式： OAI收割
来源：海洋研究所
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