Glutamate and Glutamine Enable Rapid Acclimation of Haematococcus lacustris to High-Light During Culture Phase Shift by Pre-Activating Photoprotective Pathways
文献类型：期刊论文
| 作者 | Yu, Wenjie1; Zhang, Litao1,4; Liang, Shan1,2; Liu, Jianguo1,2,3,4 |
| 刊名 | PLANT CELL AND ENVIRONMENT
 |
| 出版日期 | 2025-11-03 |
| 页码 | 15 |
| 关键词 | CO2 fixation glutamate glutamine Haematococcus lacustris phase shift photoinhibition photoprotective pathways |
| ISSN号 | 0140-7791 |
| DOI | 10.1111/pce.70255 |
| 通讯作者 | Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | Microalga Haematococcus lacustris is known for its ability to produce high-value product astaxanthin. The abrupt shift from weak-light in the vegetative phase to high-light in the astaxanthin induction phase triggers severe photodamage during the large-scale cultivation of H. lacustris for astaxanthin production in two phases, causing photobleaching with resultant biomass loss. Current mitigation relies primarily on physical shading. This study found that glutamate/glutamine supplementation before phase shift mitigated the photodamage caused by sudden high-light stress in H. lacustris. Glutamate rapidly enhanced CO2 fixation while simultaneously inducing various photoprotective pathways, including non-photochemical quenching, cyclic electron flow, chlororespiration and photorespiration. This maintained the balance between light absorption and energy utilization in H. lacustris during abrupt environmental shifts, thereby preventing damage on both the donor and acceptor sides in photosystem II. Pre-incubation with glutamate in the dark pre-activated these photoprotective pathways, thus enabling seamless acclimation to high-light stress following abrupt shifts. Meanwhile, glutamine exhibited equivalent efficacy in inducing multiple photoprotective pathways. These findings establish a novel strategy based on dark pretreatment for enhancing photosynthetic capacity under high light and protecting algal cells against photobleaching. The study provides fundamental insights into photoprotective acclimation mechanisms in microalgae and higher plants facing rapid environmental shifts. |
| WOS关键词 | CYCLIC ELECTRON FLOW ; ASTAXANTHIN ACCUMULATION ; CHLOROPHYLL FLUORESCENCE ; ALLEVIATING INHIBITION ; PHOTODAMAGED-PSII ; PLUVIALIS ; PHOTOINHIBITION ; STRESS ; PHOTORESPIRATION ; MICROALGA |
| 资助项目 | This study was supported by the National Natural Science Foundation of China (NO. 32302979 and 32370409), Natural Science Foundation of Shandong Province (NO. ZR2023QD029), China Postdoctoral Science Foundation (NO. 2023M733534), Science Technology Speci |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001605968600001 |
| 出版者 | WILEY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203725]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China 3.Natl Ctr Technol Innovat Comprehens Utilizat Salin, Academician Workstat Agr High Tech Ind Area Yellow, Dongying, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Wenjie,Zhang, Litao,Liang, Shan,et al. Glutamate and Glutamine Enable Rapid Acclimation of Haematococcus lacustris to High-Light During Culture Phase Shift by Pre-Activating Photoprotective Pathways[J]. PLANT CELL AND ENVIRONMENT,2025:15. |
| APA | Yu, Wenjie,Zhang, Litao,Liang, Shan,&Liu, Jianguo.(2025).Glutamate and Glutamine Enable Rapid Acclimation of Haematococcus lacustris to High-Light During Culture Phase Shift by Pre-Activating Photoprotective Pathways.PLANT CELL AND ENVIRONMENT,15. |
| MLA | Yu, Wenjie,et al."Glutamate and Glutamine Enable Rapid Acclimation of Haematococcus lacustris to High-Light During Culture Phase Shift by Pre-Activating Photoprotective Pathways".PLANT CELL AND ENVIRONMENT (2025):15. |
入库方式： OAI收割
来源：海洋研究所
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