Full-Length Transcriptome of Testis and Ovary Provides Insights into Alternative Splicing During Gonadal Development in Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Wang, Youyan2; Yu, Yang1,2,3; Wang, Yue1; Li, Fuhua1,3 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2025-06-19 |
| 卷号 | 26期号:12页码:16 |
| 关键词 | sexual differentiation PacBio Iso-Seq AS events gonads shrimp |
| ISSN号 | 1661-6596 |
| DOI | 10.3390/ijms26125863 |
| 通讯作者 | Yu, Yang(yuyang@qdio.ac.cn) |
| 英文摘要 | The Pacific white shrimp, Litopenaeus vannamei (L. vannamei), is an important aquaculture species, yet the molecular mechanisms underlying its sex differentiation and gonadal development remain poorly understood. A deeper understanding of these processes is critical for advancing broodstock quality and enabling unisex breeding strategies. While previous studies have focused on gene expression differences between females and males, structural differences in transcriptomic regulation between sexes have been largely overlooked. Here, we present a comprehensive full-length transcriptome analysis of L. vannamei testis and ovary, identifying 830 and 690 novel genes, respectively, and over 6000 new isoforms. Notably, we discovered extensive alternative splicing (AS) events, with the cartilage oligomeric matrix protein-like gene exhibiting over 300 AS isoforms in the ovary compared to only 2 in the testis, suggesting a potential role in ovarian development. Furthermore, sex-determining genes such as Fem-1a, Fem-1c, and Sxl were found to produce AS isoforms exclusively in ovarian tissue. We also identified three germ cell development-associated genes-MAD2-like, RAD51-like, and Su(dx)-like-that undergo distinct AS events in gonadal tissues, leading to sex-specific structural domain alterations. These findings highlight the complexity of AS-mediated post-transcriptional regulation in L. vannamei and provide novel insights into the molecular mechanisms governing sex differentiation and gonadal development. |
| WOS关键词 | DROSOPHILA SEX DETERMINATION ; ISO-SEQ ; GENES ; RNA ; LETHAL ; DIFFERENTIATION ; IDENTIFICATION ; MECHANISMS ; EXPRESSION ; EVOLUTION |
| 资助项目 | National Natural Science Foundation of China ; National Key R&D Program of China[2022YFD2400203] ; Key Research and Development Program of Shandong[2021LZGC029] ; Taishan Scholars Program ; [32441087] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001515981300001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/201973]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yu, Yang |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China 2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Youyan,Yu, Yang,Wang, Yue,et al. Full-Length Transcriptome of Testis and Ovary Provides Insights into Alternative Splicing During Gonadal Development in Litopenaeus vannamei[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2025,26(12):16. |
| APA | Wang, Youyan,Yu, Yang,Wang, Yue,&Li, Fuhua.(2025).Full-Length Transcriptome of Testis and Ovary Provides Insights into Alternative Splicing During Gonadal Development in Litopenaeus vannamei.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,26(12),16. |
| MLA | Wang, Youyan,et al."Full-Length Transcriptome of Testis and Ovary Provides Insights into Alternative Splicing During Gonadal Development in Litopenaeus vannamei".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26.12(2025):16. |
入库方式： OAI收割
来源：海洋研究所
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