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Identification and Functional Analysis of a Key Gene in the CHH Gene Family for Glucose Metabolism in the Pacific White Shrimp Litopenaeus vannamei

文献类型：期刊论文

作者Zhang, Chengyi1,2,5; Zhang, Xiaojun1,2,3,4,5; Si, Shuqing1,2,5; Sun, Mingzhe1,2,5; Li, Aixin1,2,5; Yuan, Jianbo1,2,3,4,5; Li, Fuhua1,2,3,4,5
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
出版日期2025-05-12
卷号26期号:10页码:28
关键词crustacean hyperglycemic hormone glucose metabolism alternate splicing variants growth molting
ISSN号1661-6596
DOI10.3390/ijms26104612
通讯作者Zhang, Xiaojun(xjzhang@qdio.ac.cn)
英文摘要The crustacean hyperglycemic hormone (CHH) is a unique multifunctional neuroendocrine hormone superfamily in crustaceans, crucial for maintaining physiological homeostasis and stress adaptation. To explore the role of CHHs in shrimp metabolism and growth, we identified LvCHH Ia, a CHH family member who regulates glucose metabolism in the Pacific white shrimp (Litopenaeus vannamei), through CHH family gene classification, phylogenetic analysis, gene structure analysis, and transcription factor binding site (TFBS) prediction. Subsequently, we cloned two alternative splicing variants of this gene, LvCHH Ia-1 and LvCHH Ia-2, both expressed in the nervous system but with different expression levels, and LvCHH Ia-2 exhibiting a broader tissue distribution. Using interference (RNAi)-mediated gene silencing and recombinant protein injection, we investigated the functional similarities and differences between the two variants. Our results show that both variants affect glucose metabolism by modulating the expression of key enzyme genes involved in gluconeogenesis/glycolysis, such as HK, TPI, PCK1, ALD. Specifically, they likely regulate hemolymph glucose levels via the Wnt and PI3K-AKT signaling pathways, with LvCHH Ia-1 exerting a more sustained effect on glucose metabolism compared to LvCHH Ia-2. Furthermore, LvCHH Ia may also act as a molting inhibitory hormone by suppressing the expression of ecdysone synthesis-related genes, where LvCHH Ia-2 plays a more significant role. These findings deepen our understanding of CHH regulatory mechanisms in crustaceans and provide potential applications for shrimp physiological research and aquaculture.
WOS关键词CRUSTACEAN HYPERGLYCEMIC HORMONE ; NEUROPEPTIDE ; SEQUENCE ; PROTEIN ; SUPERFAMILY ; RECRUITMENT ; PEPTIDES ; BLOOD ; VENOM ; CRAB
资助项目National Natural Sciences Foundation of China ; National Key R&D Program of China[2022YFF1000304] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Taishan Scholars Program ; [32273102]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:001496369000001
出版者MDPI
源URL[http://ir.qdio.ac.cn/handle/337002/202124]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Zhang, Xiaojun
作者单位1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Chengyi,Zhang, Xiaojun,Si, Shuqing,et al. Identification and Functional Analysis of a Key Gene in the CHH Gene Family for Glucose Metabolism in the Pacific White Shrimp Litopenaeus vannamei[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2025,26(10):28.
APA Zhang, Chengyi.,Zhang, Xiaojun.,Si, Shuqing.,Sun, Mingzhe.,Li, Aixin.,...&Li, Fuhua.(2025).Identification and Functional Analysis of a Key Gene in the CHH Gene Family for Glucose Metabolism in the Pacific White Shrimp Litopenaeus vannamei.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,26(10),28.
MLA Zhang, Chengyi,et al."Identification and Functional Analysis of a Key Gene in the CHH Gene Family for Glucose Metabolism in the Pacific White Shrimp Litopenaeus vannamei".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26.10(2025):28.

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来源：海洋研究所

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