Comparative Transcriptome Analysis Elucidates the Desiccation Stress Adaptation in Sargassum muticum
文献类型：期刊论文
| 作者 | Cao, Wei4; Zhang, Mingyi4; Wu, Nan4; Zheng, Yanxin3,4; Li, Xiaodong1,2; Han, Haiying4; Yu, Tao4; Wu, Zhongxun5; Qu, Pei3; Li, Bo4 |
| 刊名 | GENES
 |
| 出版日期 | 2025-05-16 |
| 卷号 | 16期号:5页码:14 |
| 关键词 | |
| DOI | 10.3390/genes16050587 |
| 通讯作者 | Zheng, Yanxin(zhengyanxin1989@163.com) ; Li, Bo(cdlb789@126.com) |
| 英文摘要 | Background/Objectives: Desiccation profoundly influences the distribution and abundance of intertidal seaweeds, necessitating robust molecular adaptations. Sargassum muticum is a brown seaweed inhabiting intertidal rocky substrates. During low tides, this species undergoes periodic aerial exposure. Such environmental conditions necessitate robust physiological mechanisms to mitigate desiccation stress. Yet, the molecular basis of this adaptation remains poorly understood. Methods: To investigate desiccation-responsive genes and elucidate the underlying mechanisms of adaptation, we exposed S. muticum to 6 h of controlled desiccation stress in sterilized ceramic trays, simulating natural tidal conditions, and performed comparative transcriptome analysis using RNA-seq on the Illumina NovaSeq 6000 platform. Results: High-quality sequencing identified 66,192 unigenes, with 1990 differentially expressed genes (1399 upregulated and 591 downregulated). These differentially expressed genes (DEGs) were categorized into regulatory genes-including mitogen-activated protein kinase (MAPK), calmodulin, elongation factor, and serine/threonine-protein kinase-and functional genes, such as heat shock protein family members (HSP20, HSP40, and HSP70), tubulin (TUBA and TUBB), and endoplasmic reticulum homeostasis-related genes (protein disulfide-isomerase A6, calreticulin, and calnexin). Gene Ontology (GO) enrichment highlighted upregulated DEGs in metabolic processes like glutathione metabolism, critical for oxidative stress mitigation, while downregulated genes were linked to transport functions, such as ammonium transport, suggesting reduced nutrient uptake during dehydration. KEGG pathway analysis revealed significant enrichment in "protein processing in endoplasmic reticulum" and "MAPK signaling pathway-plant", implicating endoplasmic reticulum stress response and conserved signaling cascades in desiccation adaptation. Validation via qRT-PCR confirmed consistent expression trends for key genes, reinforcing the reliability of transcriptomic data. Conclusions: These findings suggest that S. muticum undergoes extensive biological adjustments to mitigate desiccation stress, highlighting candidate pathways for future investigations into recovery and tolerance mechanisms. |
| WOS关键词 | PYROPIA-TENERA RHODOPHYTA ; HIGH-SALINITY STRESSES ; ABSCISIC-ACID ; EXPRESSION PROFILES ; CDNA MICROARRAY ; DROUGHT ; GENES ; COLD ; EXTRACTION ; TOLERANCE |
| 资助项目 | Opening foundation of the Observation and Research Station of Bohai Strait Eco-Corridor, MNR[BH202309] ; Opening foundation of the Observation and Research Station of Bohai Strait Eco-Corridor, MNR[BH202409] ; China Agriculture Research System[CARS-50] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:001495672400001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202142]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zheng, Yanxin; Li, Bo |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266000, Peoples R China 3.MNR, Observat & Res Stn Bohai Strait Ecocorridor, Qingdao 266000, Peoples R China 4.Chinese Acad Fishery Sci, Isl & Reef Fishery Res Ctr, Changdao Enhancement & Expt Stn, Yantai 264000, Peoples R China 5.Natl Ocean Pk Adm Ctr Changdao, Yantai 264000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Wei,Zhang, Mingyi,Wu, Nan,et al. Comparative Transcriptome Analysis Elucidates the Desiccation Stress Adaptation in Sargassum muticum[J]. GENES,2025,16(5):14. |
| APA | Cao, Wei.,Zhang, Mingyi.,Wu, Nan.,Zheng, Yanxin.,Li, Xiaodong.,...&Li, Bo.(2025).Comparative Transcriptome Analysis Elucidates the Desiccation Stress Adaptation in Sargassum muticum.GENES,16(5),14. |
| MLA | Cao, Wei,et al."Comparative Transcriptome Analysis Elucidates the Desiccation Stress Adaptation in Sargassum muticum".GENES 16.5(2025):14. |
入库方式： OAI收割
来源：海洋研究所
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