Identification of genes associated with resistance trait to VPAHPND in Pacific white shrimp (Litopenaeus vannamei) using Bulk Segregation Analysis (BSA)
文献类型：期刊论文
| 作者 | Bao, Zhenning1,3; Yu, Yang1,2,3; Lin, Pengfei1,3; Li, Fuhua1,2,3 |
| 刊名 | AQUACULTURE REPORTS
 |
| 出版日期 | 2025-09-15 |
| 卷号 | 43页码:10 |
| 关键词 | Litopenaeus vannamei Bulk Segregation Analysis (BSA) Resistance to VP AHPND Single nucleotide polymorphisms (SNPs) Sliding window algorithm |
| ISSN号 | 2352-5134 |
| DOI | 10.1016/j.aqrep.2025.102902 |
| 通讯作者 | Yu, Yang(yuyang@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn) |
| 英文摘要 | Vibrio parahaemolyticus harboring the PirA/PirB toxin (VPAHPND) is regarded as one of the main pathogens that caused Acute hepatopancreatic necrosis disease (AHPND) in the Pacific white shrimp Litopenaeus vannamei (L. vannamei). Breeding new varieties resistant to VPAHPND is considered to be the most effective way to reduce the economic losses in shrimp aquaculture. Identification of genes and SNP markers associated with resistance trait could accelerate the breeding efficiency by application of marker assisted selection and genomic selection. In the present study, a Bulk Segregation Analysis coupled to whole genome sequencing method was employed to identify SNPs and genes associated with resistance trait to VPAHPND in L. vannamei. The DNA of susceptible and resistant individuals was pooled separately. ED (Euclidean distance) and the chi-square test were applied to calculate the allele frequency and genotype frequency differences between susceptible and resistant individuals. A total of 2238 SNPs and 97 genes were identified to be associated with resistance trait to VPAHPND. KEGG enrichment analysis showed that these genes were significantly enriched in PPAR signaling pathway, PI3K-Akt signaling pathway, NLR pathway and O-Antigen nucleotide sugar biosynthesis pathway. The acyl-CoA delta-9 desaturase gene, 14-3-3 epsilon-like gene, retrovirus-related Pol polyprotein gene, enzymatic polyprotein gene in these pathways were considered as candidate genes associated with resistance trait to VPAHPND of shrimp. This study demonstrated that BSA based on whole genome re-sequencing was a cost-effective method for identifying disease resistant genes in aquaculture animals. These data will not only provide useful information for understanding the genetic basis of shrimp resistance trait to VPAHPND, but also offer a source of SNPs for markerassisted selection and genomic selection in shrimp breeding. |
| WOS关键词 | CLONING |
| 资助项目 | National Key R & D Program of China[2022YFD2400203] ; Key Research and Development Program of Shandong[2024LZGC038] ; Key Research and Development Program of Shandong[2021LZGC029] ; Earmarked fund[CARS-48] ; Taishan Scholars Program ; Oceanographic Data Center, IOCAS |
| WOS研究方向 | Fisheries |
| 语种 | 英语 |
| WOS记录号 | WOS:001504103800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202247]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yu, Yang; Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bao, Zhenning,Yu, Yang,Lin, Pengfei,et al. Identification of genes associated with resistance trait to VPAHPND in Pacific white shrimp (Litopenaeus vannamei) using Bulk Segregation Analysis (BSA)[J]. AQUACULTURE REPORTS,2025,43:10. |
| APA | Bao, Zhenning,Yu, Yang,Lin, Pengfei,&Li, Fuhua.(2025).Identification of genes associated with resistance trait to VPAHPND in Pacific white shrimp (Litopenaeus vannamei) using Bulk Segregation Analysis (BSA).AQUACULTURE REPORTS,43,10. |
| MLA | Bao, Zhenning,et al."Identification of genes associated with resistance trait to VPAHPND in Pacific white shrimp (Litopenaeus vannamei) using Bulk Segregation Analysis (BSA)".AQUACULTURE REPORTS 43(2025):10. |
入库方式： OAI收割
来源：海洋研究所
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