Alkaline phosphatase-coupled high affinity phosphate transporters were involved in low phosphorus stress responding in Pyropia yezoensis
文献类型：期刊论文
| 作者 | Gao, Xianrong1,5; Tian, Chao1,5; Wang, Shurui1; Guo, Menglin1; Niu, Jianfeng1,3; Wang, Guangce1,2,4 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| 出版日期 | 2025-08-01 |
| 卷号 | 90页码:11 |
| 关键词 | Pyropia yezoensis Alkaline phosphatase High-affinity symporter Pi-starvation Myb-transcription factor Dissolved organic phosphorus |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2025.104101 |
| 通讯作者 | Niu, Jianfeng(jf_niu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Phosphate (Pi) often becomes a limiting nutrient in oceans, particularly in macroalgae aquaculture areas. More importantly, the continuous increase of Pyropia yezoensis cultivation density has exacerbated Pi deficiency issues. This study used transcriptome analysis of Py. yezoensis seedlings under low-Pi conditions and identified the gene PyALP, an alkaline phosphatase, which was significantly upregulated. Through Weighted Gene Co-expression Network Analysis (WGCNA) and Pearson correlation, PyPHO89, a Pi transporter gene, and six transcription factors were co-expressed with PyALP. Bioinformatics predictions indicated that PyALP belongs to a PhoA type enzyme. However, the molecular structures of the glycosylphosphatidylinositol (GPI) and anchor the N-terminal glycine were not detected, suggesting that PyALP is confined to the microenvironment between the cell wall and plasma membrane, where it hydrolyzes dissolved organic phosphorus (DOP). PyPHO89 was predicted to be a high-affinity symporter for inorganic phosphorus uptake. Promoter analysis revealed Myb binding sites in both PyALP and PyPHO89. Among the screened potential transcription factor genes, only Py08914 possessed a conserved SHLQKYR motif and a glutamine-rich C-terminal sequence, which are characteristic of a typical Pi deficiency-responsive transcription factor. Quantitative RT-PCR showed upregulation of PyALP and PyPHO89 after 6 h of Pi-starvation, and expression of Py08914 peaked in the first 2-4 h of starvation. This implies that the hydrolysis of DOP and uptake of phosphorus are co-regulated by the Myb transcription factor. PyALP activity increased with Pi-starvation and both phosphoesters and phosphonates could be used with similar efficiency. This is the first report regarding the Pi-deficiency response mechanism in Py. yezoensis. |
| WOS关键词 | TRANSCRIPTIONAL RESPONSES ; EXPRESSION ANALYSIS ; SEQUENCE ALIGNMENT ; ACID-PHOSPHATASE ; PROTEIN ; GENE ; IDENTIFICATION ; DEFICIENCY ; LIMITATION ; STARVATION |
| 资助项目 | Major Scientific and Technological Innovation Project of Key Research and Development Program of Shandong Province[2022LZGC004] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Natural Science Foundation of Qingdao[24-4-4-zrjj-145-jch] ; Natural Science Foundation of Shandong Province[ZR2024MD011] ; Agriculture Research System of China of MOF and MARA[CARS-50] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001504556300001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202253]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Niu, Jianfeng; Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266000, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266000, Peoples R China 3.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Genet Resources & Breeding, Lianyungang 222005, Peoples R China 4.Laoshan Lab, Qingdao 266237, Peoples R China 5.Qingdao Agr Univ, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xianrong,Tian, Chao,Wang, Shurui,et al. Alkaline phosphatase-coupled high affinity phosphate transporters were involved in low phosphorus stress responding in Pyropia yezoensis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2025,90:11. |
| APA | Gao, Xianrong,Tian, Chao,Wang, Shurui,Guo, Menglin,Niu, Jianfeng,&Wang, Guangce.(2025).Alkaline phosphatase-coupled high affinity phosphate transporters were involved in low phosphorus stress responding in Pyropia yezoensis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,90,11. |
| MLA | Gao, Xianrong,et al."Alkaline phosphatase-coupled high affinity phosphate transporters were involved in low phosphorus stress responding in Pyropia yezoensis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 90(2025):11. |
入库方式： OAI收割
来源：海洋研究所
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