Effect of nitrogen limitation and carbon sources on hydrogen and lipid production by Chlorella pyrenoidosa
文献类型：期刊论文
| 作者 | Zhang, Weiqing1,4; Dong, Shibin2; Zhang, Litao1,5; Yu, Wenjie1,5; Li, Ling1,5; Tsygankov, Anatoly3; Liu, Jianguo1,5 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2025-10-01 |
| 卷号 | 91页码:11 |
| 关键词 | Chlorella pyrenoidosa Hydrogen production Lipid accumulation Nitrogen limitation Carbon sources |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2025.104128 |
| 通讯作者 | Li, Ling(liling@qdio.ac.cn) ; Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | Biofuel (including H2 and lipids) from green algae is an attractive way to produce renewable energy. Under nitrogen-limitation, Chlorella pyrenoidosa produced both H2 and lipids. In this study, the effects of nitrogen and carbon sources on the growth and the co-production of H2 and lipids in C. pyrenoidosa were examined, and the regulatory mechanisms including photosynthetic electron transport, NADPH production and acetyl-CoA metabolism were investigated. The peak H2 yield (241.42 mL L- 1) was found in 5 % nitrogen concentration (0.35 mM NH4Cl) treatment, with the total lipid content being 45.0 % of dry weight. Nitrogen-limitation decreased PSII activity, inhibiting photosynthetic O2 evolution, and thereby creating favorable anaerobic conditions for enhanced H2 production. Excess energy in the photosynthetic electron transport chain prompted hydrogenase to consume surplus electrons as the terminal electron acceptor and produce H2. Furthermore, nitrogen-limitation induced overreduction on the PSII acceptor side, resulting in the accumulation of NADPH, which provided reducing power for fatty acids synthesis. The addition of acetic acid enhanced the growth, H2 production, and lipid accumulation in C. pyrenoidosa, with a recommended concentration of 2 mL L- 1. It was proposed that acetic acid inhibited photosynthesis, subsequently decreasing photosynthetic O2 evolution. Additionally, acetic acid was converted into acetyl-CoA to provide the precursor for fatty acid synthesis, and it also promoted the generation of NADH, which provided the reducing power required for both fatty acid synthesis and H2 production. These results highlight the positive roles of nitrogen limitation and acetic acid in enhancing H2 and lipid production in C. pyrenoidosa and provide a basis for improving Chlorella's energy production efficiency through artificial regulation. |
| WOS关键词 | DEPRIVED CHLAMYDOMONAS-REINHARDTII ; MARINE MICROALGA ; PHOTOPRODUCTION ; ACID ; ENHANCEMENT ; CULTIVATION ; ADAPTATION ; PROTOTHECOIDES ; ACCUMULATION ; STARVATION |
| 资助项目 | Qingdao New Energy Shandong Laboratory Open Project[QNESL OP202311] ; National Natural Science Foundation of China[32072945] ; National Natural Science Foundation of China[41676142] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001508678900001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202334]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Ling; Liu, Jianguo |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 2.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China 3.Russian Acad Sci, Pushchino Sci Ctr Biol Res, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia 4.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao 266400, Peoples R China 5.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weiqing,Dong, Shibin,Zhang, Litao,et al. Effect of nitrogen limitation and carbon sources on hydrogen and lipid production by Chlorella pyrenoidosa[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2025,91:11. |
| APA | Zhang, Weiqing.,Dong, Shibin.,Zhang, Litao.,Yu, Wenjie.,Li, Ling.,...&Liu, Jianguo.(2025).Effect of nitrogen limitation and carbon sources on hydrogen and lipid production by Chlorella pyrenoidosa.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,91,11. |
| MLA | Zhang, Weiqing,et al."Effect of nitrogen limitation and carbon sources on hydrogen and lipid production by Chlorella pyrenoidosa".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 91(2025):11. |
入库方式： OAI收割
来源：海洋研究所
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