Transition between germination and dormancy for non-motile cells of Haematococcus pluvialis: dependence on nitrogen availability through metabolic flux of amino acids and nucleotides
文献类型：期刊论文
| 作者 | Li, Jing2,4; Zhang, Litao1,2; Yu, Wenjie1,2; Chen, Feng2,4; Tsygankov, Anatoly A.3; Liu, Jianguo1,2,5 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2025-10-01 |
| 卷号 | 434页码:10 |
| 关键词 | Nitrogen assimilation Reactive oxygen species Purine Pyrimidine |
| ISSN号 | 0960-8524 |
| DOI | 10.1016/j.biortech.2025.132818 |
| 通讯作者 | Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | The slow growth and time-consuming cultivation process of Haematococcus pluvialis hinder its large-scale production. Germination of red, non-motile cells presents a novel strategy to improve cultivation efficiency; however, the underlying metabolic adaptations remain poorly understood. This study reveals the regulatory role of nitrogen in the dormancy-to-germination transition. Upon nitrogen supplementation, dormant cells initially reactivated respiration, followed by the restoration of photosynthesis. By contrast, nitrogen deficiency restricted zoospore release and caused carbon accumulation by limited nitrogen assimilation and impaired mitochondrial respiration. Notably, nitrogen deficiency disrupted nucleotide balance by reducing levels of amino acids and purine nucleotides while increasing pyrimidines (uracil, uridine monophosphate, orotate), ultimately impeding DNA replication. Moreover, reactive oxygen species may function as signaling molecules to trigger metabolic reactivation during germination. These findings highlight nitrogen as a central regulator of cell cycle progression in H. pluvialis, offering possible insights into the population dynamics of eukaryotic microalgae with dormant life stages. |
| WOS关键词 | ASTAXANTHIN ACCUMULATION ; SODIUM-ACETATE ; PHOTORESPIRATION ; DEGRADATION ; PYRIMIDINE ; CULTURE ; PURINE ; WALL |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; TS Industrial Experts Program[LJNY202102] ; National Natural Science Foundation of China[32370409] ; National Natural Science Foundation of China[32302979] ; Science & Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta[2022SZX12] ; Yunnan Science and Technology Talent Development[2] ; Yunnan Science and Technology Talent Development[81] |
| WOS研究方向 | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:001512901800003 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202375]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, 168 Wenhai Rd, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, 88 Haijun Rd, Qingdao 266404, Peoples R China 3.RAS, Inst Basic Biol Problems, Pushchino 142290, Moscow Region, Russia 4.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao 266404, Peoples R China 5.Natl Ctr Technol Innovat Comprehens Utilizat Salin, Academician Workstat Agr High Tech Ind Area Yellow, Dongying 257300, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jing,Zhang, Litao,Yu, Wenjie,et al. Transition between germination and dormancy for non-motile cells of Haematococcus pluvialis: dependence on nitrogen availability through metabolic flux of amino acids and nucleotides[J]. BIORESOURCE TECHNOLOGY,2025,434:10. |
| APA | Li, Jing,Zhang, Litao,Yu, Wenjie,Chen, Feng,Tsygankov, Anatoly A.,&Liu, Jianguo.(2025).Transition between germination and dormancy for non-motile cells of Haematococcus pluvialis: dependence on nitrogen availability through metabolic flux of amino acids and nucleotides.BIORESOURCE TECHNOLOGY,434,10. |
| MLA | Li, Jing,et al."Transition between germination and dormancy for non-motile cells of Haematococcus pluvialis: dependence on nitrogen availability through metabolic flux of amino acids and nucleotides".BIORESOURCE TECHNOLOGY 434(2025):10. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。