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SsGSDMEa-mediated pyroptosis is involved in Photobacterium damselae subsp. piscicida infection in the black rockfish Sebastes schlegelii

文献类型：期刊论文

作者Ding, Xin1,4,5; Chen, Yuan1,4,5; Yuan, Zihao1,2,5; Zhang, Jian2,3; Li, Xuepeng3; Xu, Hang1; Sun, Li2,4,5
刊名AQUACULTURE
出版日期2025-10-15
卷号609页码:11
关键词Sebastes schlegelii Photobacterium damselae subsp. piscicida Pyroptosis Gasdermin Caspase
ISSN号0044-8486
DOI10.1016/j.aquaculture.2025.742828
通讯作者Xu, Hang(xuhang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
英文摘要Pyroptosis is a necrotic cell death mediated by gasdermin, which is activated by caspase (CASP) cleavage. CASP also mediates apoptosis, a non-necrotic cell death. Photobacterium damselae subsp. piscicida (PDP) is a bacterial pathogen that causes necrotic cell death in fish, including black rockfish Sebastes schlegelii, but the underlying mechanism, especially that associated with pyroptosis, remains unclear. Here, using S. schlegelii as a representative species, we examined the involvement of gasdermin-mediated pyroptosis in PDP infection in fish. We identified two gasdermin E (GSDME) orthologs, SsGSDMEa and SsGSDMEb, from S. schlegelii and found that only SsGSDMEa could trigger pyroptosis. We also identified three S. schlegelii CASPs (SsCASP3, 9, and 10), all of which induced apoptosis. SsGSDMEa was cleaved by SsCASP3 at 261NVVD264, producing an N-terminal fragment (NT) named NT264. SsGSDMEa was also cleaved by SsCASP 9 and 10 in the same manner at 203IESD206 and 246IEAD249, producing NT206 and NT249, respectively. NT264 and NT249, but not NT206, triggered pyroptosis. PDP infection upregulated SsGSDMEa and SsCASP3/9/10 expressions in fish tissues and activated SsGSDMEa and SsCASP3/9/ 10 in fish cells, leading to pyroptosis. Taken together, the findings deepened our understanding of the CASPmediated activation mechanism of black rockfish GSDME and its involvement in pathogen infection.
WOS关键词EXPRESSION ANALYSIS ; NLRP3 INFLAMMASOME ; CELL-DEATH ; PROTEIN ; IDENTIFICATION ; GASDERMINS ; CLEAVAGE ; FAMILY ; SKIN
资助项目National Natural Science Foundation of China[41576150] ; National Natural Science Foundation of China[U24A20463]
WOS研究方向Fisheries ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:001511524100006
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/202403]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xu, Hang; Sun, Li
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Yantai Univ, Sch Ocean, Yantai, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Xin,Chen, Yuan,Yuan, Zihao,et al. SsGSDMEa-mediated pyroptosis is involved in Photobacterium damselae subsp. piscicida infection in the black rockfish Sebastes schlegelii[J]. AQUACULTURE,2025,609:11.
APA Ding, Xin.,Chen, Yuan.,Yuan, Zihao.,Zhang, Jian.,Li, Xuepeng.,...&Sun, Li.(2025).SsGSDMEa-mediated pyroptosis is involved in Photobacterium damselae subsp. piscicida infection in the black rockfish Sebastes schlegelii.AQUACULTURE,609,11.
MLA Ding, Xin,et al."SsGSDMEa-mediated pyroptosis is involved in Photobacterium damselae subsp. piscicida infection in the black rockfish Sebastes schlegelii".AQUACULTURE 609(2025):11.

入库方式： OAI收割

来源：海洋研究所

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