Enhanced urea competitiveness in Pyropia yezoensis via PyDUR3.1 overexpression: Infeasibility of reducing P. yezoensis aquaculture area in green tide control
文献类型：期刊论文
| 作者 | Guo, Menglin2,4,5; Zheng, Zhenbing4,5; Gu, Wenhui4,5; Huan, Li4,5; Wang, Xulei4,5; Wang, Wenjun3; Wang, Hui4,5; Wang, Guangce1,4,5 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2025-08-01 |
| 卷号 | 90页码:10 |
| 关键词 | Green tide DUR3.1 Pyropia yezoensis Urea competitiveness Phycoerythrin |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2025.104190 |
| 通讯作者 | Zheng, Zhenbing(zbzheng@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Since 2007, large-scale green tides have occurred in both the Yellow Sea and the Bohai Sea of China. In particular, the green tide in the Yellow Sea has been consistently associated with Ulva prolifera. However, current research on U. prolifera itself has not effectively achieved the source control of green tide. Green tide outbreaks in the Yellow Sea have been hypothesized to originate from U. prolifera spores attaching to Pyropia yezoensis aquaculture rafts in the Subei Shoal. During the early stage of bloom formation, P. yezoensis and U. prolifera may coexist and compete for dissolved organic nitrogen within the same aquaculture environment. In recent years, coastal waters have shown a gradual increase in urea content, providing additional organic nitrogen that can contribute to harmful algal blooms, posing a threat to coastal ecosystems. Therefore, understanding the differential nitrogen absorption characteristics of these two species is crucial for mitigating green tide outbreaks. DUR3.1 is responsible for high-affinity transport of exogenous urea, although research on this protein in macroalgae is limited. This study revealed different urea absorption strategies between P. yezoensis and U. prolifera, with U. prolifera requiring less urea to achieve greater biomass accumulation. Results demonstrate that over-expressing PyDUR3.1 significantly enhances exogenous urea nitrogen absorption in P. yezoensis, with absorption rates increasing by up to 133 %. This leads to a notable inhibition of U. prolifera fresh weight increase under nitrogen-limiting conditions, with a reduction of 82 %. The findings suggest that P. yezoensis aquaculture plays a beneficial role in mitigating green tide outbreaks. In contrast, reducing the area of P. yezoensis aquaculture, a strategy commonly used for green tide prevention, does not effectively address the issue at its source. Overall, this study provides mechanistic evidence that enhancing urea utilization in P. yezoensis could contribute to green tide mitigation, offering a new perspective on bloom prevention strategies that go beyond targeting U. prolifera itself. |
| WOS关键词 | ULVA-PROLIFERA IMPLICATIONS ; YELLOW SEA ; NITROGEN ; TEMPERATURE ; ASSIMILATION ; CULTIVATION ; EXPANSION ; CHINA ; ALGAE ; BLOOM |
| 资助项目 | National Natural Science Foundation of China[42106157] ; Foundation of State Key Laboratory of Mariculture Biobreeding and Sustainable Goods[BRESG202308] ; Opening Foundation of Key Laboratory of Breeding Biotechnology and Sustain-able Aquaculture, CAS[2023FB12] ; Major Scientific and Techno-logical Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; Natural Science Foundation of Qingdao[24-4-4-zrjj-145-jch] ; Key Technology Research and Development Projects of Qingdao[25-1-1-gjgg-54-h] ; Shandong Province Natural Science Foundation[ZR2024MD011] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Nan-tong City Social and Livelihood Science and Technology Program[MS2023008] ; China Agriculture Research System of MOF[CARS-50] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001534852800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202659]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zheng, Zhenbing; Wang, Guangce |
| 作者单位 | 1.Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Menglin,Zheng, Zhenbing,Gu, Wenhui,et al. Enhanced urea competitiveness in Pyropia yezoensis via PyDUR3.1 overexpression: Infeasibility of reducing P. yezoensis aquaculture area in green tide control[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2025,90:10. |
| APA | Guo, Menglin.,Zheng, Zhenbing.,Gu, Wenhui.,Huan, Li.,Wang, Xulei.,...&Wang, Guangce.(2025).Enhanced urea competitiveness in Pyropia yezoensis via PyDUR3.1 overexpression: Infeasibility of reducing P. yezoensis aquaculture area in green tide control.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,90,10. |
| MLA | Guo, Menglin,et al."Enhanced urea competitiveness in Pyropia yezoensis via PyDUR3.1 overexpression: Infeasibility of reducing P. yezoensis aquaculture area in green tide control".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 90(2025):10. |
入库方式： OAI收割
来源：海洋研究所
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