A Type Ia Crustin from the Pacific White Shrimp Litopenaeus vannamei Exhibits Antimicrobial and Chemotactic Activities
文献类型：期刊论文
| 作者 | Gao, Xiuyan2,3; Liu, Yuan1,2; Huang, Xiaoyang2; Yang, Zhanyuan2; Sun, Mingzhe2; Li, Fuhua1,2 |
| 刊名 | BIOMOLECULES
 |
| 出版日期 | 2025-07-14 |
| 卷号 | 15期号:7页码:18 |
| 关键词 | |
| DOI | 10.3390/biom15071015 |
| 通讯作者 | Liu, Yuan(liuyuan@qdio.ac.cn) |
| 英文摘要 | Crustins are a family of cysteine-rich antimicrobial peptides (AMPs), predominantly found in crustaceans, and play important roles in innate immunity. However, among the many reported crustins, few studies have explored their immunomodulatory functions. In this study, we investigated the immune function of a type I crustin (LvCrustinIa-2) in Litopenaeus vannamei, with particular emphasis on comparing the roles of its different domains. LvCrustinIa-2 possesses cationic patchy surface and amphipathic structure, and its expression was significantly induced in hemocytes after pathogen challenge. Both the recombinant LvCrustinIa-2 (rLvCrustinIa-2) and its whey acidic protein (WAP) domain (rLvCrustinIa-2-WAP) exhibited significant inhibitory activities against the tested Gram-positive bacteria. They also showed binding affinity not only for Gram-positive bacteria but also for Gram-negative bacteria. Furthermore, rLvCrustinIa-2 induced membrane leakage and structure damage in the target bacteria. Notably, chemotaxis assays revealed that rLvCrustinIa-2 and the synthetic cysteine-rich region (LvCrustinIa-2-CR) significantly enhanced the chemotactic activity of shrimp hemocytes in vitro. Knockdown of LvCrustinIa-2 triggered significant transcriptional activation of genes involved in calcium transport, inflammation, redox regulation, and NF-kappa B pathway. Taken together, these findings elucidate the distinct roles of the cysteine-rich region and WAP domain in type Ia crustin and provide the first evidence of a crustacean AMP with chemotactic and immunomodulatory activities. |
| WOS关键词 | CHINESE MITTEN CRAB ; MOLECULAR CHARACTERIZATION ; ANTIBACTERIAL ACTIVITIES ; MAMMALIAN DEFENSINS ; PEPTIDES ; EXPRESSION ; CRAYFISH ; IMMUNITY ; PROTEIN ; HEMOCYTES |
| 资助项目 | National Natural Science Foundation of China ; Taishan Scholars Program ; [32273172] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001535528700001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202728]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Yuan |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China 3.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Xiuyan,Liu, Yuan,Huang, Xiaoyang,et al. A Type Ia Crustin from the Pacific White Shrimp Litopenaeus vannamei Exhibits Antimicrobial and Chemotactic Activities[J]. BIOMOLECULES,2025,15(7):18. |
| APA | Gao, Xiuyan,Liu, Yuan,Huang, Xiaoyang,Yang, Zhanyuan,Sun, Mingzhe,&Li, Fuhua.(2025).A Type Ia Crustin from the Pacific White Shrimp Litopenaeus vannamei Exhibits Antimicrobial and Chemotactic Activities.BIOMOLECULES,15(7),18. |
| MLA | Gao, Xiuyan,et al."A Type Ia Crustin from the Pacific White Shrimp Litopenaeus vannamei Exhibits Antimicrobial and Chemotactic Activities".BIOMOLECULES 15.7(2025):18. |
入库方式： OAI收割
来源：海洋研究所
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