Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses
文献类型：期刊论文
| 作者 | Zhang, Xintang1,3,4; Xu, Fei1,3,4; Hu, Xiaoli2; Meng, Jie2 |
| 刊名 | MARINE ENVIRONMENTAL RESEARCH
 |
| 出版日期 | 2025-10-01 |
| 卷号 | 211页码:13 |
| 关键词 | Oyster Zn/Cu antagonism Accumulation Antioxidant system Immune responses |
| ISSN号 | 0141-1136 |
| DOI | 10.1016/j.marenvres.2025.107393 |
| 通讯作者 | Xu, Fei(xufei@qdio.ac.cn) ; Meng, Jie(mengjie86@ouc.edu.cn) |
| 英文摘要 | To elucidate the interaction between zinc (Zn) and copper (Cu), this study investigates the mechanisms by which Zn mediates detoxification of Cu toxicity in the Pacific oyster Crassostrea gigas. Physiological indices and transcriptome analyses under control (CTR), Cu, and Zn_Cu treatments reveal that excess Cu induces systemic toxicity in oysters. Cu accumulation in tissues alters metal transporters, downregulating Cu importers and exporters (Zn and Cu transporters) while upregulating chaperones and metallothionein (MT1) for detoxification. Zn supplementation alleviates these effects by enhancing MT1 expression, blocking Cu uptake, and restoring metal homeostasis. Cu stress also increases reactive oxygen species (ROS) and disrupts antioxidant responses, with superoxide dismutase (SOD) overactivation and suppression of catalase1 (CAT1) and glutathione peroxidase (GPX2), leading to potential H2O2 accumulation. Zn supplementation reduces oxidative stress by restoring CAT1 and GPX2 expression, promoting glutathione metabolism, thus maintaining redox balance. Furthermore, Cu suppresses immune signaling by downregulating TLR-MyD88-NF-kappa B pathway genes and promotes apoptosis through caspase3/7 activation and reduced anti-apoptotic gene expression. Zn partially reverses these effects by reactivating immune genes and reducing caspase levels, though Cu's immune suppression remains dominant. In conclusion, at the tested concentrations (100 mu g/L Cu and 50 mu g/L Zn) and Zn/Cu ratio, Zn supplementation mitigated Cu-induced oxidative stress and immune suppression in hemocytes, demonstrating its potential for mitigating Cu toxicity in aquaculture. |
| WOS关键词 | MECHANISMS ; APOPTOSIS ; METALLOTHIONEINS ; DETOXIFICATION ; EXPOSURE ; CELLS ; SEA |
| 资助项目 | National Key Research and Development Program of China[2024YFD2402800] ; National Natural Science Foundation of China[32373157] ; Taishan Scholars Program[tsqn202211250] ; Key Research and Development Program of Shandong[2022LZGC015] ; Oceanographic Data Center, Institute of Oceanology, Chinese Academy of Sciences (IOCAS) |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:001544947800001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202773]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xu, Fei; Meng, Jie |
| 作者单位 | 1.Univ Chinese Acad Sci, Shenyang, Peoples R China 2.Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266000, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xintang,Xu, Fei,Hu, Xiaoli,et al. Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses[J]. MARINE ENVIRONMENTAL RESEARCH,2025,211:13. |
| APA | Zhang, Xintang,Xu, Fei,Hu, Xiaoli,&Meng, Jie.(2025).Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses.MARINE ENVIRONMENTAL RESEARCH,211,13. |
| MLA | Zhang, Xintang,et al."Zinc mitigates copper toxicity in Crassostrea gigas by regulating metal homeostasis, oxidative stress, and immune responses".MARINE ENVIRONMENTAL RESEARCH 211(2025):13. |
入库方式： OAI收割
来源：海洋研究所
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