Systems metabolic engineering of Escherichia coli for the bioproduction of biliverdin and phycoerythrobilin
文献类型：期刊论文
| 作者 | Li, Shuang2,3,5,6; Lu, Chang4; Zang, Xiaonan1; Duan, Delin2,5,6; Shao, Zhanru2,5,6 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2025-07-25 |
| 卷号 | 16页码:8 |
| 关键词 | biliverdin phycoerythrobilin heterologous expression biosynthesis neoporphyra haitanensis |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2025.1640158 |
| 通讯作者 | Shao, Zhanru(zrshao@qdio.ac.cn) |
| 英文摘要 | Phycobiliprotein is an important co-pigment in photosynthesis, which is composed of the covalent combination of apoprotein and phycobilin. Biliverdin IX alpha and phycoerythrobilin are both important substances in the phycobiliprotein biosynthesis pathway. As an economic red seaweed, Neoporphyra haitanensis has a high content of phycoerythrin. Therefore, in this study, we explored new enzyme resources for the heterologous biosynthesis of biliverdin and phycoerythrobilin. Specifically, we identified and isolated the genes encoding NhHO1, NhPebA and NhPebB from N. haitanensis, which are integral components of its phycoerythrin biosynthetic pathway. Additionally, ApHO1 from Arthrospira platensis and PebS from Prochlorococcus phage P-SSM2 were selected for comparative analysis. The results showed that genes from N. haitanensis did not encode active enzymes, which might be ascribed to the absence of crucial motifs. However, the transformation of ApHO1 and PebS into Escherichia coli could lead to the synthesis of biliverdin and phycoerythrobilin. This is the first report of sequence analysis and enzyme activity verification of phycoerythrin synthesis genes from N. haitanensis, providing a foundation for future explorations into its potential genetic resources. The successful production of biliverdin and phycoerythrobilin lay a foundation for the environmentally friendly preparation of phycobiliprotein. |
| WOS关键词 | HEME OXYGENASE ; BIOSYNTHESIS ; PHYCOBILISOME ; EXTRACTION ; EXPRESSION ; ENZYMES ; CLONING ; GENE |
| 资助项目 | Qingdao International Collaboration Project[22-3-6-ghgg-1-hz] ; Qingdao West Coast New District University Presidents Fund[E42424101N] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001545717600001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202788]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Shao, Zhanru |
| 作者单位 | 1.Ocean Univ China, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao, Shandong, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Yantai Univ, Sch Life Sci, Yantai Key Lab Characterist Agr Bioresource Conser, Yantai, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 6.Chinese Acad Sci, CAS, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shuang,Lu, Chang,Zang, Xiaonan,et al. Systems metabolic engineering of Escherichia coli for the bioproduction of biliverdin and phycoerythrobilin[J]. FRONTIERS IN PLANT SCIENCE,2025,16:8. |
| APA | Li, Shuang,Lu, Chang,Zang, Xiaonan,Duan, Delin,&Shao, Zhanru.(2025).Systems metabolic engineering of Escherichia coli for the bioproduction of biliverdin and phycoerythrobilin.FRONTIERS IN PLANT SCIENCE,16,8. |
| MLA | Li, Shuang,et al."Systems metabolic engineering of Escherichia coli for the bioproduction of biliverdin and phycoerythrobilin".FRONTIERS IN PLANT SCIENCE 16(2025):8. |
入库方式： OAI收割
来源：海洋研究所
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