Mutant library construction and green fluorescent protein expression in a marine fungus capable of plastics biodegradation
文献类型：期刊论文
| 作者 | Liu, Rui1,5,6; Wang, Yani1,4,5; Su, Zhenjie1,4,5; Yang, Jun1,3; Fei, Fan1,4,5; Gao, Rongrong2; Sun, Chaomin1,5,6 |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2025-08-18 |
| 页码 | 14 |
| 关键词 | mutant library
green fluorescent protein
plastics biodegradation
|
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-025-5049-5 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
| 英文摘要 | With the increasing accumulation of plastic pollutants in various environments, research on microorganisms (including bacteria, fungi, and algae) with plastic degradation capabilities has gained significant attention. However, only a limited number of microbial plastic-degrading enzymes have been identified to date. This highlights that the degradation mechanisms employed by many plastic-degrading microorganisms, particularly filamentous fungi, remain insufficiently explored. In this study, we utilized a versatile fungal plasmid (pCT74) to express green fluorescent protein (GFP) in a marine-derived fungus Alternaria alternata strain FB1 with plastic degradation capabilities. Upon evaluating the degradation effect of polyester-type polyurethane (PU) film, we observed that different transformants exhibited three kinds of activities (the same, reduced, or enhanced degradation capability) compared to the FB1 wild-type strain. Further analysis of the plasmid fragment insertion sites in different transformants revealed that pCT74 integrates randomly into the genome of the host fungus. Notably, a direct correlation was found between the plasmid insertion site and the degradation capability of the corresponding transformant. Our findings not only redefine the potential applications of plasmid pCT74 in filamentous fungi but also show a novel research approach to identifying key enzymes involved in plastic degradation by fungi. |
| WOS关键词 | ASYMMETRIC INTERLACED PCR ; AGROBACTERIUM-TUMEFACIENS ; SCLEROTINIA-SCLEROTIORUM ; GENE-EXPRESSION ; COLONIZATION ; TRANSFORMATION ; AMPLIFICATION ; REPORTER |
| 资助项目 | Science and Technology Innovation Project of Laoshan Laboratory[2022QNLM030004-3] ; Science and Technology Innovation Project of Laoshan Laboratory[LSKJ202203103] ; NSFC Innovative Group Grant[42221005] ; Key Collaborative Research Program of the Alliance of International Science Organizations[ANSO-CR-KP-2022-08] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Major Research Plan of the National Natural Science Foundation[92351301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Scholars Program[tstp20230637] ; Qingdao Natural Science Foundation[23-2-1-182-zyyd-jch] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001551639700001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203152]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Yantai Vocat Coll, Yantai 264670, Peoples R China 3.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Rui,Wang, Yani,Su, Zhenjie,et al. Mutant library construction and green fluorescent protein expression in a marine fungus capable of plastics biodegradation[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2025:14. |
| APA | Liu, Rui.,Wang, Yani.,Su, Zhenjie.,Yang, Jun.,Fei, Fan.,...&Sun, Chaomin.(2025).Mutant library construction and green fluorescent protein expression in a marine fungus capable of plastics biodegradation.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14. |
| MLA | Liu, Rui,et al."Mutant library construction and green fluorescent protein expression in a marine fungus capable of plastics biodegradation".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2025):14. |
入库方式： OAI收割
来源：海洋研究所
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