The involvement of caspase-8-3 in cleavaging caspase-3 and immune response of oyster Crassostrea gigas
文献类型：期刊论文
| 作者 | Yang, Chuanyan2,3; Xu, Jiachao1; Tong, Ziling2,3; Wang, Xiudan1; Jiang, Shuai1; Yang, Bin1; Wang, Lingling2,3; Song, Linsheng2,3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2025-11-01 |
| 卷号 | 166页码:10 |
| 关键词 | Crassostrea gigas Caspase-8-3 Caspase-3 Apoptosis Immune response |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2025.110677 |
| 通讯作者 | Wang, Lingling(wanglingling@dlou.edu.cn) |
| 英文摘要 | The caspase family play a crucial role in both apoptosis signaling and immune response. In the present study, a caspase-8 was identified from Crassostrea gigas (designed as CgCaspase-8-3), which contained two tandem death effector domains (DEDs) and a CASc domain, as well as conserved cysteine active site motif "QACQG". CgCaspase-8-3 was initially clustered with molluscan Caspase-8 homologs, and subsequently grouped with vertebrate sequences, while maintaining a relatively distant phylogenetic relationship from the mammalian clade. It was widely expressed in various tissues with the highest expression level in mantle, as well as in different development stages with the highest expression level in two-cell embryos. CgCaspase-8-3 was localized in the cytoplasm and co-localized with CgCaspase-3 in haemocytes. Although the full-length recombinant CgCaspase-8-3 showed weak proteolytic activity toward Ac-IETD-pNA, its C-terminal truncated form (rCgCaspase-8-3(CT)) displayed enhanced enzymatic activity and efficiently cleaved the downstream effector rCgCaspase-3 in vitro, indicating a direct role in the caspase activation cascade. This activity was significantly inhibited by the caspase8-specific inhibitor Z-IETD-FMK. In addition, CgCaspase-8-3 mRNA expression in haemocytes significantly upregulated post LPS, PGN and Vibrio splendidus stimulation. All these results collectively indicate that CgCaspase-8-3 is involved in apoptosis and immune response in oyster. |
| WOS关键词 | TOLL-LIKE RECEPTORS ; NF-KAPPA-B ; MOLECULAR-MECHANISMS ; APOPTOSIS ; GENE ; LIPOPOLYSACCHARIDE ; EXPRESSION ; MUSSELS ; MEMBERS ; FAMILY |
| 资助项目 | National Science Foundation of China[32230110] ; Fund for CARS-49 ; Outstanding Talents and Innovative Teams of Agricultural Scientific Research in MARA ; Innovation team of Aquaculture Environment Safety from Liaoning Province[LT202009] ; Dalian High Level Talent Innovation Support Program[2022RG14] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001565959800001 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203302]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lingling |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Control, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Chuanyan,Xu, Jiachao,Tong, Ziling,et al. The involvement of caspase-8-3 in cleavaging caspase-3 and immune response of oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2025,166:10. |
| APA | Yang, Chuanyan.,Xu, Jiachao.,Tong, Ziling.,Wang, Xiudan.,Jiang, Shuai.,...&Song, Linsheng.(2025).The involvement of caspase-8-3 in cleavaging caspase-3 and immune response of oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,166,10. |
| MLA | Yang, Chuanyan,et al."The involvement of caspase-8-3 in cleavaging caspase-3 and immune response of oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 166(2025):10. |
入库方式： OAI收割
来源：海洋研究所
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