The Atlas of the Shell Proteome in Oysters Reveals the Potential Roles of the Cytoskeleton and Extracellular Matrix in Biomineralization
文献类型：期刊论文
| 作者 | Yang, Qi1,7; Wang, Shentong1,3,5; Liu, Mingkun1,4,5,6; Wang, Wei1,3,5; Zhang, Guofan1,2,4,5; Li, Li1,2,3,4,5,7 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2025-09-08 |
| 页码 | 12 |
| 关键词 | biomineralization oyster shell matrix protein |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.5c00671 |
| 通讯作者 | Li, Li(lili@qdio.ac.cn) |
| 英文摘要 | Shell matrix proteins (SMPs) are fundamental biological macromolecules for mollusk shell formation, yet fewer than 400 SMPs in mollusks have been previously identified, hindering our understanding of how mollusks construct and maintain their shells. Here, we identified 1689 SMPs in the Pacific oyster Crassostrea gigas using three different mass spectrometry techniques, representing a significant methodological advancement in shell proteomics, enabling a 6.52-fold increase in SMP identification compared to previous studies. Gene ontology and domain annotation revealed cytoskeletal proteins (with cofilin ADF, tubulin, and myosin head domains) and extracellular matrix (ECM)-related proteins (with carbonic anhydrase, chitin-binding, von Willebrand type A, and EGF domains) as the key functional SMPs involved in biomineralization. Furthermore, developmental transcriptomics highlighted that microtubule- and microfilament-related SMPs were enriched in larvae and adults, respectively, potentially reflecting differences in cytoskeletal regulation associated with larval aragonitic and adult calcitic shells. Transcriptomic analyses revealed that acidifying stress significantly downregulated the expression of genes encoding collagen and stress-fiber-related proteins, while activating the BMP signaling pathway in oysters. These transcriptional changes suggest a potential impairment in ECM and cytoskeletal maintenance. Our findings indicate the potential roles of the cytoskeleton and ECM proteins in biomineralization and emphasize the complexity of biological controls on shell formation in oysters. Furthermore, the proteomic strategy combining three distinct technologies can be applied to other mollusks and provide deeper insights into their evolutionary trajectories under future environmental changes. |
| WOS关键词 | CARBONIC-ANHYDRASE ; CELL MECHANICS ; PROTEINS ; CALCITE ; MANTLE ; IDENTIFICATION ; ACIDIFICATION ; ARAGONITE |
| 资助项目 | National Key Research and Development Program of China[2023YFD2401102] ; National Natural Science Foundation of China[42406096] ; Key Research and Development Program of Shandong[ZFJH202309] ; Department of Science & Technology of Shandong Province[2022GJJLJRC02-096] ; China Agriculture Research System of MOF and MARA[CARS-49] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001566318400001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203308]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 4.Shandong Ctr Technol Innovat Oyster Seed Ind, Qingdao 266000, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 6.Southern Marine Sci & Engn Guangdong Lab Zhanjiang, Oyster Ind Technol Inst Zhanjiang, Zhanjiang 523936, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Qi,Wang, Shentong,Liu, Mingkun,et al. The Atlas of the Shell Proteome in Oysters Reveals the Potential Roles of the Cytoskeleton and Extracellular Matrix in Biomineralization[J]. JOURNAL OF PROTEOME RESEARCH,2025:12. |
| APA | Yang, Qi,Wang, Shentong,Liu, Mingkun,Wang, Wei,Zhang, Guofan,&Li, Li.(2025).The Atlas of the Shell Proteome in Oysters Reveals the Potential Roles of the Cytoskeleton and Extracellular Matrix in Biomineralization.JOURNAL OF PROTEOME RESEARCH,12. |
| MLA | Yang, Qi,et al."The Atlas of the Shell Proteome in Oysters Reveals the Potential Roles of the Cytoskeleton and Extracellular Matrix in Biomineralization".JOURNAL OF PROTEOME RESEARCH (2025):12. |
入库方式： OAI收割
来源：海洋研究所
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