A novel indel-based molecular sex identification system for oplegnathus fasciatus: Insights from ndc80 gene polymorphisms and
文献类型：期刊论文
| 作者 | Xiao, Yongshuang2,3; Xu, Pingrui1,2,3; Li, Jun2,3; Xiao, Zhizhong2,3 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
 |
| 出版日期 | 2025-12-01 |
| 卷号 | 56页码:7 |
| 关键词 | Oplegnathus fasciatus ndc80 gene Intron region Sex molecular marker |
| ISSN号 | 1744-117X |
| DOI | 10.1016/j.cbd.2025.101632 |
| 通讯作者 | Xiao, Yongshuang(yongshuangxiao@qdio.ac.cn) |
| 英文摘要 | Oplegnathus fasciatus is a commercially important marine fish species, valued both in wild fisheries and aquaculture. It possesses a multivalent sex-determination system (X1X1X2X2/X1X2Y) and exhibits marked sexual growth dimorphism, with males demonstrating significantly faster growth rates. Sex-specific molecular markers are instrumental in advancing selective breeding strategies. In this study, whole-genome screening of O. fasciatus revealed a male-specific structural variant within an intronic region of ndc80: two insertions of 3 bp and 538 bp (totaling 541 bp), which were consistently absent in females. To facilitate practical application, we developed a PCR-based assay using a single primer pair that amplifies a conserved region flanking the insertion site. This assay reproducibly generates distinct banding patterns: males yield two fragments (208 bp and 749 bp), consistent with the 541 bp male-specific insertion, whereas females yield only the 208 bp amplicon. This method enables efficient, high-throughput sex identification in O. fasciatus without the need for sequencing. Using standard agarose gel electrophoresis, the assay reliably distinguishes sexes through clearly divergent banding patterns. Beyond applications in selective breeding, these sex-specific markers provide critical molecular insights into the sex determination mechanisms and the genetic basis of sexual dimorphism in O. fasciatus. |
| WOS关键词 | NDC80 COMPLEX ; MARKERS ; PROGNOSIS ; ALIGNMENT ; SEQUENCE |
| 资助项目 | National Natural Science Foundation of China[42276107] ; Natural Science Foundation of Shandong Province[ZR2024MC071] ; Shandong Province Key R D Program[2023LZGCQY001] ; Asia Collaboration Project on the Devel-opment of Ecological Marine Ranching ; National Key Research and Development Program[2022YFC3103600] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:001574019700001 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203398]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xiao, Yongshuang |
| 作者单位 | 1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Yongshuang,Xu, Pingrui,Li, Jun,et al. A novel indel-based molecular sex identification system for oplegnathus fasciatus: Insights from ndc80 gene polymorphisms and[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2025,56:7. |
| APA | Xiao, Yongshuang,Xu, Pingrui,Li, Jun,&Xiao, Zhizhong.(2025).A novel indel-based molecular sex identification system for oplegnathus fasciatus: Insights from ndc80 gene polymorphisms and.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,56,7. |
| MLA | Xiao, Yongshuang,et al."A novel indel-based molecular sex identification system for oplegnathus fasciatus: Insights from ndc80 gene polymorphisms and".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 56(2025):7. |
入库方式： OAI收割
来源：海洋研究所
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