Molecular characteristics of gonadal differentiation dimorphism in the ovoviviparous fish Sebastes schlegelii under gradient high-temperature induction
文献类型：期刊论文
| 作者 | Huang, Jinwei2,4,5; Xiao, Yongshuang3,4,5; Song, Zongcheng1; Li, Jun3,4,5 |
| 刊名 | MARINE POLLUTION BULLETIN
 |
| 出版日期 | 2026 |
| 卷号 | 222页码:12 |
| 关键词 | Thermal stress Sex differentiation Transcriptome Metabolome Climate change adaptation |
| ISSN号 | 0025-326X |
| DOI | 10.1016/j.marpolbul.2025.118717 |
| 通讯作者 | Xiao, Yongshuang(yongshuangxiao@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | Global warming can alter fish sex ratios and threaten population stability, particularly in species with temperature-sensitive sex differentiation. Sebastes schlegelii, an ovoviviparous species adapted to both cold and warm waters, may exhibit feminization or masculinization under high temperatures. However, the underlying molecular mechanisms remain unclear. We hypothesized that high-temperature exposure activates distinct sex-differentiation pathways and sought to identify the key genes and metabolites involved. Our findings reveal a balanced sex ratio at 18 degrees C, a notable feminization at 24 degrees C with 83.3 % of individuals being female, and a significant masculinization at 27 degrees C where 76.7 % are male. Transcriptomic and metabolomic analyses revealed at 24 degrees C, pathways related to steroid hormone biosynthesis, estrogen signaling, and progesterone, androgen, and estrogen receptor agonists/antagonists were activated, whereas at 27 degrees C, activation was prominent in steroid hormone biosynthesis, steroid biosynthesis, and cholesterol metabolism. Key genes and metabolites included cyp7a1, cyp1a1, hsd17b1, hsd17b2, er alpha, creb1, hsp70, and Estrone at 24 degrees C, and cyp7a1, lipa, lss, and Taurocholic acid at 27 degrees C. Quantitative reverse transcription PCR (RT-qPCR) revealed upregulation of female differentiation genes (foxl2, cyp19a1) at 24 degrees C and male differentiation genes (sox9, amh, dmrt1, hsd11b2) at 27 degrees C. Hormonal assays showed elevated 17 beta-estradiol (E2) at 24 degrees C and 11-ketotestosterone (11-KT) at 27 degrees C. In situ hybridization (ISH) revealed that, in stage II gonads, sox9 and amh are predominantly localized in Sertoli cells of the testis. These findings support our hypothesis and demonstrate that high temperature drives sex differentiation in S. schlegelii through distinct molecular programs, identifying core regulators and pathways underlying temperature-sensitive sexual development. |
| WOS关键词 | SEX DETERMINATION ; MARINE ; REVERSAL ; GENES |
| 资助项目 | National Key Research and Develop-ment Program[2024YFD2400102] ; National Natural Science Founda-tion of China[42276107] ; Natural Science Foundation of Shandong Province[ZR2024MC071] ; Asia Collaboration Project on the Develop-ment of Ecological Marine Ranching |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001576917000001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203413]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xiao, Yongshuang; Li, Jun |
| 作者单位 | 1.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Jinwei,Xiao, Yongshuang,Song, Zongcheng,et al. Molecular characteristics of gonadal differentiation dimorphism in the ovoviviparous fish Sebastes schlegelii under gradient high-temperature induction[J]. MARINE POLLUTION BULLETIN,2026,222:12. |
| APA | Huang, Jinwei,Xiao, Yongshuang,Song, Zongcheng,&Li, Jun.(2026).Molecular characteristics of gonadal differentiation dimorphism in the ovoviviparous fish Sebastes schlegelii under gradient high-temperature induction.MARINE POLLUTION BULLETIN,222,12. |
| MLA | Huang, Jinwei,et al."Molecular characteristics of gonadal differentiation dimorphism in the ovoviviparous fish Sebastes schlegelii under gradient high-temperature induction".MARINE POLLUTION BULLETIN 222(2026):12. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。