In situ Raman spectroscopic relative quantitative analysis of sulfur metabolic dynamics in deep-sea microorganisms
文献类型：期刊论文
| 作者 | He, Wanying4; Cai, Ruining1,2,5; Guo, Xiaoxiao4; Zhang, Yitong2,3,5; Li, Lianfu4; Xi, Shichuan4; Du, Zengfeng2,3,5; Luan, Zhendong2,3,5; Zhuo, Jintao2,3,5; Sun, Chaomin1,2,5 |
| 刊名 | MICROBIOLOGY SPECTRUM
 |
| 出版日期 | 2025-09-25 |
| 页码 | 11 |
| 关键词 | quantitative analysis microorganisms metabolic process confocal Raman microscopy sulfur cycle |
| DOI | 10.1128/spectrum.02059-25 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn) |
| 英文摘要 | Deciphering dynamic regulation of microbial sulfur metabolism in deep-sea environments is critical for understanding global biogeochemical cycles and climate feedback mechanisms. Current analytical approaches face limitations in achieving quantitative, in situ visualization of microbial metabolic processes, including susceptibility to environmental interference during sampling and analysis, leading to impaired data accuracy. This study developed an innovative method based on confocal Raman spectroscopy utilizing nitrogen as an internal standard for metabolite quantification. Taking sulfate, which is a major component of seawater and essential for the sulfur cycle, as a model, we quantified it in solid medium and monitored the in situ metabolic processes of deep-sea Erythrobacter flavus 21-3. The non-invasive technique revealed previously unrecognized light-dependent differences in microbial metabolic patterns between deep-sea and laboratory conditions through spectral visualization and relative quantification. We found that natural light exposure promoted sulfate production and enhanced zero-valent sulfur (cyclooctasulfur S8) accumulation near the surface, accompanied by co-enrichment of carotenoids, suggesting the presence of light-driven sulfur metabolic processes. In contrast, dark conditions favored S8 storage in the subsurface layers, potentially supported by abundant internal organic carbon sources as energy reserves. These findings may provide new insights into photo-regulated sulfur transformation mechanisms. Our approach establishes an analytical framework for in situ quantitative investigation of microbially mediated elemental cycling processes.IMPORTANCEMicrobial sulfur metabolism in the deep ocean is critical to global biogeochemical cycles, yet its regulatory mechanisms remain poorly understood, largely due to methodological limitations. In this study, we introduce an innovative non-invasive, quantitative approach using confocal Raman spectroscopy with molecular nitrogen (N2) as an internal standard, overcoming major obstacles in real-time metabolic monitoring. Our results demonstrate light-dependent adaptations in sulfur metabolism among deep-sea bacteria, unveiling previously unrecognized photo-regulated sulfur transformations that refine our understanding of microbial ecological strategies in these environments. The established analytical framework provides a versatile platform for in situ investigation of microbial-driven elemental cycling across diverse extreme ecosystems. |
| WOS关键词 | HYDROTHERMAL VENT ; CARBON-DIOXIDE ; SULFATE ; PROBE |
| 资助项目 | National Natural Science Foundation of China[42327805] ; National Natural Science Foundation of China[42221005] ; Key Project of the Ocean Research Center, Chinese Academy of Sciences[COMS2020J03] ; China Postdoctoral Science Foundation[2024M751269] ; Shandong Provincial Natural Science Foundation[ZR2024QD074] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001578367600001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203419]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Chaomin; Zhang, Xin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Key Lab Ocean Observat & Forecasting, Key Lab Marine Geol & Environm, Qingdao, Peoples R China 4.Laoshan Lab, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Wanying,Cai, Ruining,Guo, Xiaoxiao,et al. In situ Raman spectroscopic relative quantitative analysis of sulfur metabolic dynamics in deep-sea microorganisms[J]. MICROBIOLOGY SPECTRUM,2025:11. |
| APA | He, Wanying.,Cai, Ruining.,Guo, Xiaoxiao.,Zhang, Yitong.,Li, Lianfu.,...&Zhang, Xin.(2025).In situ Raman spectroscopic relative quantitative analysis of sulfur metabolic dynamics in deep-sea microorganisms.MICROBIOLOGY SPECTRUM,11. |
| MLA | He, Wanying,et al."In situ Raman spectroscopic relative quantitative analysis of sulfur metabolic dynamics in deep-sea microorganisms".MICROBIOLOGY SPECTRUM (2025):11. |
入库方式： OAI收割
来源：海洋研究所
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