Binding Stability and Catalytic Mechanism of a Novel Chitin Deacetylase from Cytobacillus firmus and Preparation of Chitosan
文献类型：期刊论文
| 作者 | Liang, Bicheng1,4; Zhang, Tong1,4; Li, Guantian1; Yang, Haoyue1; Wang, Zongji2; Li, Kecheng1,3; Liu, Song1,3; Yu, Huahua1,3; Xing, Ronge1,3 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2025-10-01 |
| 卷号 | 73期号:39页码:24866-24881 |
| 关键词 | chitin
chitin deacetylase
|
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.5c08532 |
| 通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) |
| 英文摘要 | This study reports the heterologous expression, purification, and functional characterization of a novel chitin deacetylase (CDA) from Cytobacillus firmus. The recombinant CfCDA exhibited optimal activity at pH 7 and 35 degrees C, with Mg2+ enhancing its function. Deep eutectic solvent (DES) pretreatment significantly improved chitin accessibility, enabling CfCDA to produce chitosan with a high degree of deacetylation (71.2%) and recovery rate (14.2%). Structural analyses confirmed reduced crystallinity and successful acetyl removal. Molecular docking and dynamics simulations identified Asp318, His470, and Trp433 as key catalytic residues with stable binding to (GlcNAc)4. These findings highlight CfCDA's potential for eco-friendly chitosan production and provide a basis for future enzyme engineering. |
| WOS关键词 | BY-PRODUCTS ; PURIFICATION ; BIOCONVERSION ; CARBOHYDRATE ; OPTIMIZATION ; ACETYLATION ; PROTEIN |
| 资助项目 | National Natural Science Foundation of China[2022CXGC020413] ; Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China ; Taishan Industrial Experts Program[42276097] ; National Natural Science Foundation of China[ZR2022YQ37] ; Natural Science Foundation of Shandong Province[CARS-49] ; Modern Agro-industry Technology Research System[2024ZY0039] ; Central Guidance for Local Scientific and Technological Development Funds[25-1-1-186-zyyd-jch] ; Qingdao Natural Science Foundation |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:001576267600001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203428]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xing, Ronge |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266000, Peoples R China 2.Linyi Univ, Regenerat Med Innovat Inst, Linyi 276000, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Bicheng,Zhang, Tong,Li, Guantian,et al. Binding Stability and Catalytic Mechanism of a Novel Chitin Deacetylase from Cytobacillus firmus and Preparation of Chitosan[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2025,73(39):24866-24881. |
| APA | Liang, Bicheng.,Zhang, Tong.,Li, Guantian.,Yang, Haoyue.,Wang, Zongji.,...&Xing, Ronge.(2025).Binding Stability and Catalytic Mechanism of a Novel Chitin Deacetylase from Cytobacillus firmus and Preparation of Chitosan.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,73(39),24866-24881. |
| MLA | Liang, Bicheng,et al."Binding Stability and Catalytic Mechanism of a Novel Chitin Deacetylase from Cytobacillus firmus and Preparation of Chitosan".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73.39(2025):24866-24881. |
入库方式： OAI收割
来源：海洋研究所
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