中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Genome-wide identification and analysis of the PLP_deC genes involved in taurine synthesis in bivalves

文献类型：期刊论文

作者Pang, Meiqian4; Qi, Haigang1,3,4,5; Wang, Min4; Du, Mingyang4; Chen, Jincheng4; Cong, Rihao1,3,4,5; Li, Li1,2,3,4,5; Zhang, Guofan1,2,3,5
刊名COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
出版日期2025-12-01
卷号56页码:10
关键词Bivalves Pyridoxal phosphate-dependent decarboxylase Cysteine sulfinic acid decarboxylase Glutamate decarboxylase-like 1 Taurine
ISSN号1744-117X
DOI10.1016/j.cbd.2025.101641
通讯作者Qi, Haigang(qihaigang@qdio.ac.cn) ; Li, Li(lili@qdio.ac.cn)
英文摘要Bivalves, such as oysters and scallops, are rich in taurine and play crucial roles in marine ecosystems as well as in aquaculture. However, mechanisms governing the taurine biosynthesis in bivalves remain poorly understood. Cysteine sulfinic acid decarboxylase (CSAD) and glutamate decarboxylase-like 1 (GADL1), as members of pyridoxal phosphate-dependent decarboxylase (PLP_deC) family, catalyze the decarboxylation step in taurine synthesis. To investigate their evolution and function in bivalves, a genome-wide identification of these genes was conducted in five bivalve species. A total of 61 PLP_deC genes were identified, of which 23 were predicted to be involved in taurine synthesis. Phylogenetic analysis revealed that these genes cluster into two distinct groups: CSAD/GADL1-like and GAD-like. Notably, bivalves possess only one Gad gene, in contrast to the two typically found in vertebrates, whereas they harbor three to five Csad/Gadl1 genes, compared with just two in vertebrates. Three bivalve Csad genes exhibited high expression levels across most of developmental stages and adult tissues, suggesting their essential roles in development and the maintenance of normal physiological activities. Molecular docking analysis revealed that oyster CSADs exhibit higher substrate-binding specificity for cysteine sulfinic acid, while GAD shows higher specificity for glutamate. Heterologous overexpression assays demonstrated that oyster CSAD and GAD can significantly increase cellular taurine levels. This study is the first to provide evidence of the expansion of Csad genes in bivalve genomes and their involvement in taurine synthesis, offering novel insights into understanding the molecular mechanisms underlying the high taurine content in bivalves.
WOS关键词SULFINIC ACID DECARBOXYLASE ; GLUTAMATE-DECARBOXYLASE ; MOLECULAR EVOLUTION ; PURIFICATION ; ALIGNMENT ; PATTERNS ; SYSTEM ; GADL1 ; KS ; KA
资助项目National Natural Science Foundation of China[42376095] ; National Key Research and Development Program of China[2022YFD2400301] ; Key Research and Development Program (Agricultural Seed Improvement Project) of Shandong Province[2024LZGCQY003] ; Oceanographic Data Center, Institute of Oceanology, Chinese Academy of Sciences ; Earmarked fund[CARS-49]
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
语种英语
WOS记录号WOS:001582074000001
出版者ELSEVIER SCIENCE INC
源URL[http://ir.qdio.ac.cn/handle/337002/203467]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Qi, Haigang; Li, Li
作者单位1.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266404, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266404, Peoples R China
3.Shandong Ctr Technol Innovat Oyster Seed Ind, Qingdao 266105, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266404, Peoples R China
5.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Pang, Meiqian,Qi, Haigang,Wang, Min,et al. Genome-wide identification and analysis of the PLP_deC genes involved in taurine synthesis in bivalves[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2025,56:10.
APA Pang, Meiqian.,Qi, Haigang.,Wang, Min.,Du, Mingyang.,Chen, Jincheng.,...&Zhang, Guofan.(2025).Genome-wide identification and analysis of the PLP_deC genes involved in taurine synthesis in bivalves.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,56,10.
MLA Pang, Meiqian,et al."Genome-wide identification and analysis of the PLP_deC genes involved in taurine synthesis in bivalves".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 56(2025):10.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。