Systematic analysis of Wnt family genes reveals the potential role of Wnt4 in driving female differentiation in Spotted knifejaw
文献类型：期刊论文
| 作者 | Zhao, Haixia1,2; Xiao, Yongshuang2,3,4; Xiao, Zhizhong2,3,4; Li, Jun2,3; Xu, Shihong2,3; Jiang, Wei5 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2025-10-20 |
| 卷号 | 785页码:11 |
| 关键词 | Genome-wide Wnt family Spotted knifejaw Female differentiation wnt4 |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2025.152684 |
| 通讯作者 | Xiao, Yongshuang(yongshuangxiao@qdio.ac.cn) ; Jiang, Wei(jiangwei@fio.org.cn) |
| 英文摘要 | Wnt family genes encode secreted glycoproteins that regulate cell proliferation, differentiation, and organ growth. While much research has focused on species with standard chromosomal systems, there is a lack studies on marine fish species like Spotted knifejaw (Oplegnathus punctatus), which possess a multiple sex chromosome system (X1X1X2X2/X1X2Y). We employed a combination of whole-genome scanning of the Wnt family, and qPCR analyses, RNAi knockdown to assess the specific contribution of wnt4 to female sexual development. We identified 16 Wnt genes in the Spotted knifejaw, with OpWnt2, OpWnt3, OpWnt4, OpWnt7, OpWnt8, OpWnt9, and OpWnt10 exhibiting two genotypes in females, while OpWnt4 and OpWnt9 had only one sequence in males. Phylogenetic analysis grouped the Wnt family into three clusters: OpWnt2/OpWnt5, OpWnt1/OpWnt6/OpWnt4, and OpWnt3/OpWnt8/OpWnt10/OpWnt7/OpWnt9/OpWnt11/OpWnt16. All members of the Wnt family contain a Wnt1 domain. Wnt4 showed higher expression in female ovaries (5-40 dph) compared to males and may act upstream of foxl2, which also regulates female differentiation. Chromosomal localization of Opwnt4 was found on female chromosome 3 and male heterozygous chromosome 1. Knockdown wnt4 upregulated male-biased genes (amh, dmrt1, sox9a) while enhancing female-biased genes (foxl2, cyp19a, cyp19b), indicating that Opwnt4 synergistically regulates female differentiation and antagonizes male pathways. This study provides new insights into wnt4's role in sexual differentiation and lays the foundation for future research on sex chromosome systems in marine fish. |
| WOS关键词 | BETA-CATENIN ; EXPRESSION ; IDENTIFICATION ; SOX9 ; SRY ; POLARITY ; PATHWAY ; CLONING ; INT-1 |
| 资助项目 | National Natural Science Foundation of China[42276107] ; Natural Science Foundation of Shandong Province[ZR2024MC071] ; Natural Science Foundation of Shandong Province[ZR2025QC1413] ; Shandong Province Key RD Program[2023LZGCQY001] ; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment[FEEL202504] ; Asia Collaboration Project on the Development of Ecological Marine Ranching ; R & D Project of Marine Science Research Institute of Shandong Province[2024SDMSRI0423] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:001582528200001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203518]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xiao, Yongshuang; Jiang, Wei |
| 作者单位 | 1.Natl Oceanog Ctr, Marine Sci Res Inst Shandong Prov, Lab Benthic Fisheries Aquaculture & Enhancement, Shandong Key Lab Intelligent Marine Ranch Under Pr, Qingdao 266104, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Minist Nat Resources Peoples Republ China, Inst Oceanog 1, Observat & Res Stn Bohai Ecocorridor, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Haixia,Xiao, Yongshuang,Xiao, Zhizhong,et al. Systematic analysis of Wnt family genes reveals the potential role of Wnt4 in driving female differentiation in Spotted knifejaw[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2025,785:11. |
| APA | Zhao, Haixia,Xiao, Yongshuang,Xiao, Zhizhong,Li, Jun,Xu, Shihong,&Jiang, Wei.(2025).Systematic analysis of Wnt family genes reveals the potential role of Wnt4 in driving female differentiation in Spotted knifejaw.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,785,11. |
| MLA | Zhao, Haixia,et al."Systematic analysis of Wnt family genes reveals the potential role of Wnt4 in driving female differentiation in Spotted knifejaw".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 785(2025):11. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。