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Global immunostimulatory mechanisms of a high-branched glycogen from Glyptocidaris crenularis eggs in RAW264.7 macrophages via BONCAT-based nascent proteomics

文献类型：期刊论文

作者Wang, Qingchi5,6; Luo, Yuhang3,6; Xin, Yi4,6; Jin, Yuanxiang2,6; Yang, Guiling6; Zhang, Quanbin1; Wen, Zhengshun6
刊名CARBOHYDRATE POLYMERS
出版日期2025-12-15
卷号370页码:16
关键词Sea urchin Glycogen BONCAT Nascent proteomics Immunostimulatory mechanism
ISSN号0144-8617
DOI10.1016/j.carbpol.2025.124473
通讯作者Zhang, Quanbin(qbzhang@qdio.ac.cn) ; Wen, Zhengshun(wenzhengshun@xhlab.ac.cn)
英文摘要Marine polysaccharides have emerged as promising immunomodulators. However, conventional analytical approaches have failed to elucidate their activation mechanisms from a global perspective. In this study, we isolated a highly branched glycogen (GCEP2a) from eggs of the sea urchin Glyptocidaris crenularis. Structural characterization revealed that GCEP2a possesses a backbone composed of-*4)-alpha-D-Glcp-(1-* residues, with one C6-branch per five residues, and a molecular weight of 8.06 x 103 kDa. Employing nascent-proteome bioorthogonal non-canonical amino acid tagging (BONCAT) enrichment followed by DIA-FAIMS, we unraveled the global immunostimulatory mechanisms of GCEP2a in RAW264.7 macrophages. This advanced approach enabled dynamic profiling of nascent protein synthesis, uncovering a multi-receptor and multi-pathway regulatory network. Specifically, GCEP2a activates signaling consistent with engagement of the toll-like receptors (TLRs), Ctype lectin receptors (CLRs), and RIG-I-like receptors (RLRs), thereby triggering downstream signaling cascades including the NF-kappa B, MAPK, and PI3K-AKT pathways. Additionally, GCEP2a downregulates the biosynthesis of lacto-and neolacto-series glycosphingolipids, indicating metabolic reprogramming associated with M1 polarization. These findings not only suggest that GCEP2a exhibits potential as an immune adjuvant, but also provide initial insights into the application of BONCAT-based nascent proteomics for investigating the multi-modal immunostimulatory mechanisms of marine polysaccharides.
WOS关键词BETA-GLUCAN ; STRUCTURAL-CHARACTERIZATION ; IMMUNOMODULATORY ACTIVITY ; ANTITUMOR-ACTIVITY ; POLYSACCHARIDE ; IDENTIFICATION
资助项目National Natural Science Foundation of China[32401061] ; Natural Science Foundation of Shandong Province[ZR2022QB116] ; Key R&D Program of Zhejiang[2025C02088]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001587985900005
出版者ELSEVIER SCI LTD
源URL[http://ir.qdio.ac.cn/handle/337002/203563]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Zhang, Quanbin; Wen, Zhengshun
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310032, Zhejiang, Peoples R China
3.Ocean Univ China, Coll Food Sci & Engn, State Key Lab Marine Food Proc & Safety Control, Qingdao 266400, Peoples R China
4.Changchun Univ, Sch Food Sci & Engn, Changchun 130022, Peoples R China
5.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Dept Food Sci & Nutr, Lab Food Proc & Engn, Hangzhou 310058, Peoples R China
6.Xianghu Lab, Hangzhou 311231, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Qingchi,Luo, Yuhang,Xin, Yi,et al. Global immunostimulatory mechanisms of a high-branched glycogen from Glyptocidaris crenularis eggs in RAW264.7 macrophages via BONCAT-based nascent proteomics[J]. CARBOHYDRATE POLYMERS,2025,370:16.
APA Wang, Qingchi.,Luo, Yuhang.,Xin, Yi.,Jin, Yuanxiang.,Yang, Guiling.,...&Wen, Zhengshun.(2025).Global immunostimulatory mechanisms of a high-branched glycogen from Glyptocidaris crenularis eggs in RAW264.7 macrophages via BONCAT-based nascent proteomics.CARBOHYDRATE POLYMERS,370,16.
MLA Wang, Qingchi,et al."Global immunostimulatory mechanisms of a high-branched glycogen from Glyptocidaris crenularis eggs in RAW264.7 macrophages via BONCAT-based nascent proteomics".CARBOHYDRATE POLYMERS 370(2025):16.

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来源：海洋研究所

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