Transgenic approaches to boost bromoform production in Pyropia yezoensis for application as anti-methanogenic feed additive
文献类型：期刊论文
| 作者 | Zheng, Zhenbing1,2,3; Gu, Wenhui1,2,3; Guo, Menglin1,2,3; Wang, Lijun1,2,3; Wang, Xulei1,2,3; Huan, Li1,2,3; Wang, Guangce1,2,3 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2025-12-01 |
| 卷号 | 92页码:8 |
| 关键词 | Bromoform Vanadium bromoperoxidase Pyropia yezoensis Asparagopsis taxiformis Transgene |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2025.104322 |
| 通讯作者 | Gu, Wenhui(wenhuigu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Methane is a potent greenhouse gas, and reducing its emissions from ruminants is a global environmental priority. The red macroalga Asparagopsis taxiformis is rich in bromoform, a compound with strong antimethanogenic properties, but its large-scale cultivation faces technical and ecological challenges. In contrast, Pyropia yezoensis is an economically important red alga that is widely and efficiently cultivated, yet its natural bromoform content is lower than that of A. taxiformis, limiting its application as a feed additive for methane mitigation. In this study, we aimed to enhance bromoform synthesis in P. yezoensis through genetic engineering. The key genes mbb1 and mbb2 from A. taxiformis, involved in bromoform biosynthesis, were cloned, codonoptimized, and expressed in P. yezoensis. Additionally, the endogenous PyBPO1 gene, encoding vanadium bromoperoxidase, was overexpressed. Transgenic strains exhibited a significant but transient increase in bromoform production, with most of the synthesized bromoform released into the culture medium. However, sustained highlevel bromoform synthesis was not achieved due to transgene silencing, likely caused by co-suppression and feedback regulation. Our findings also suggest that substrate availability is a limiting factor for bromoform biosynthesis in P. yezoensis. This work demonstrates the feasibility of using genetic modification to enhance bromoform production in P. yezoensis, but also highlights the need to overcome transgene silencing and optimize metabolic pathways for stable, long-term production. These results provide a foundation for developing macroalgal feed additives to reduce methane emissions from ruminants. |
| WOS关键词 | RED ALGA ; BROMOPEROXIDASE ; DEFENSE |
| 资助项目 | Foundation of State Key Laboratory of Mariculture Biobreeding and Sustainable Goods[BRESG202308] ; National Key Research and Development Program of China[2023YFD2401102] ; National Key Research and Development Program of China[2024YFB4206602] ; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; Natural Science Foundation of Qingdao[24-4-4-zrjj-145-jch] ; Shandong Province Natural Science Foundation[ZR2024MD011] ; National Natural Science Foundation of China[42376091] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; China Agricultural Research System of MOF[CARS-50] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001594385100001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203578]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Gu, Wenhui; Wang, Guangce |
| 作者单位 | 1.Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Zhenbing,Gu, Wenhui,Guo, Menglin,et al. Transgenic approaches to boost bromoform production in Pyropia yezoensis for application as anti-methanogenic feed additive[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2025,92:8. |
| APA | Zheng, Zhenbing.,Gu, Wenhui.,Guo, Menglin.,Wang, Lijun.,Wang, Xulei.,...&Wang, Guangce.(2025).Transgenic approaches to boost bromoform production in Pyropia yezoensis for application as anti-methanogenic feed additive.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,92,8. |
| MLA | Zheng, Zhenbing,et al."Transgenic approaches to boost bromoform production in Pyropia yezoensis for application as anti-methanogenic feed additive".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 92(2025):8. |
入库方式： OAI收割
来源：海洋研究所
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