Comparative Proteomics Analysis Reveals Differential Immune Responses of Paralichthys olivaceus to Edwardsiella tarda Infection Under High and Low Temperature
文献类型：期刊论文
| 作者 | Chen, Xiaojuan1; Luo, Lejia1; Zhang, Beibei3; Zhou, Xiaowei1; Zhang, Kaipeng1; Zhang, Panpan4; Sun, Bin2,5 |
| 刊名 | BIOLOGY-BASEL
 |
| 出版日期 | 2025-10-15 |
| 卷号 | 14期号:10页码:21 |
| 关键词 | |
| DOI | 10.3390/biology14101417 |
| 通讯作者 | Zhang, Beibei(zhangbeibei@qau.edu.cn) ; Sun, Bin(sunbin@faas.cn) |
| 英文摘要 | Simple Summary The health of farmed marine fish, including the Japanese flounder (Paralichthys olivaceus), is severely compromised by temperature fluctuations and infections such as Edwardsiella tarda, resulting in significant economic losses. A previous study revealed that elevated temperature enhanced E. tarda dissemination in flounder tissues. However, the underlying mechanism has not been fully explained, especially the changes in protein level. This study utilized label-free proteomics to investigate the immune response of flounder to E. tarda infection under low (15 degrees C) and high (23 degrees C) temperature conditions. Multiple differentially abundant proteins (DAPs) were identified in each group. GO and KEGG analyses highlighted immune-related pathways and proteins, with key DAPs forming extensive interaction networks. Venn analysis revealed distinct responses: high temperature promoted endocytosis and complement activation, while low temperature increased histone levels and impaired RNA transport. These results enhance our understanding of how water temperature changes affect antibacterial immunity in fish.Abstract Fluctuating water temperatures and bacterial pathogens such as Edwardsiella tarda pose a serious threat to mariculture, resulting in significant economic losses within the flounder industry. A previous study revealed that elevated temperature enhanced E. tarda dissemination in flounder tissues. However, the underlying mechanism has not been fully explained, especially the changes in protein level. In this study, label-free proteomics was utilized to investigate the impact of high temperature (23 degrees C) and low temperature (15 degrees C) on flounder immune response to E. tarda infection. Our results identified 317 differentially abundant proteins (DAPs) in the low-temperature group (LI-LC) and 302 DAPs in the high-temperature group (HI-HC). GO and KEGG analyses of DAPs revealed numerous immune-related proteins and pathways. Twenty-six key DAPs in the LI-LC group and twenty-seven key DAPs in the HI-HC group were further identified and formed extensive interaction networks, respectively. Through the analysis of key immune-related DAPs that were specifically identified in both groups via Venn diagram analysis, we demonstrated that the endocytosis capacity and complement activity were enhanced in the HI-HC group, while histone abundance and RNA transport function were, respectively, increased and severely interfered with in the LI-LC group. These findings highlight a clear divergence in the immune response of flounder to E. tarda infection between 15 degrees C and 23 degrees C, providing valuable insights into how temperature variation influences antibacterial immunity in fish. |
| WOS关键词 | OLIVE FLOUNDER ; ENVIRONMENTAL-TEMPERATURE ; ANTIMICROBIAL ACTIVITY ; COMPLEMENT ACTIVATION ; NILE TILAPIA ; EXPRESSION ; PROTEIN ; STRESS ; SURFACE ; IDENTIFICATION |
| 资助项目 | Key Laboratory of Marine Biotechnology of Fujian Province ; Natural Science Foundation of Fujian Province[2024J08233] ; Young and Middle-aged Teacher Education Research Project of Fujian Province[JAT220075] ; Young and Middle-aged Teacher Education Research Project of Fujian Province[JAT231072] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001601826400001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203680]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Beibei; Sun, Bin |
| 作者单位 | 1.Fujian Agr & Forestry Univ, Coll Marine Sci, State Key Lab Mariculture Breeding, Key Lab Marine Biotechnol Fujian Prov, Fuzhou 350002, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China 4.Hainan Univ, State Key Lab Marine Resource Utilizat South China, Haikou 570228, Peoples R China 5.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xiaojuan,Luo, Lejia,Zhang, Beibei,et al. Comparative Proteomics Analysis Reveals Differential Immune Responses of Paralichthys olivaceus to Edwardsiella tarda Infection Under High and Low Temperature[J]. BIOLOGY-BASEL,2025,14(10):21. |
| APA | Chen, Xiaojuan.,Luo, Lejia.,Zhang, Beibei.,Zhou, Xiaowei.,Zhang, Kaipeng.,...&Sun, Bin.(2025).Comparative Proteomics Analysis Reveals Differential Immune Responses of Paralichthys olivaceus to Edwardsiella tarda Infection Under High and Low Temperature.BIOLOGY-BASEL,14(10),21. |
| MLA | Chen, Xiaojuan,et al."Comparative Proteomics Analysis Reveals Differential Immune Responses of Paralichthys olivaceus to Edwardsiella tarda Infection Under High and Low Temperature".BIOLOGY-BASEL 14.10(2025):21. |
入库方式： OAI收割
来源：海洋研究所
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