Targeting marine jellyfish toxins: development of metalloproteinase inhibitors through a specific method for protease substrate identification
文献类型：期刊论文
| 作者 | Yu, Chunlin2,3; Wang, Zhanhua2; Wang, Wenjie1,4; Li, Rongfeng1,4; Xing, Ronge1,4; Liu, Song1,4; Li, Pengcheng1,4; Yu, Huahua1,4 |
| 刊名 | ARCHIVES OF TOXICOLOGY
 |
| 出版日期 | 2025-10-29 |
| 页码 | 18 |
| 关键词 | |
| ISSN号 | 0340-5761 |
| DOI | 10.1007/s00204-025-04223-9 |
| 通讯作者 | Yu, Huahua(yuhuahua@qdio.ac.cn) |
| 英文摘要 | The venom of hazardous jellyfish contains harmful substances to human health, such as metalloproteinases, which are challenging to purify. Therefore, identifying substrate sequences using crude venom is crucial for the development of peptide inhibitors. This study utilized a designed peptide library and an abundance-based hydrolysis product analysis approach to identify the substrate sequences of toxin metalloproteinases from Nemopilema nomurai nematocyst venom (NnNV) and further modified these sequences into peptide inhibitors. Specifically, a peptide library comprising 41 potential substrate sequences was constructed and incubated with NnNV. 12 substrate sequences and 14 cleavage sites were identified from the hydrolysis products. In the design of peptide inhibitors, thiol (-SH) and phosphate groups (-PO3H2) were used as zinc-binding groups, and sixty derived peptides were obtained. 15 peptide inhibitors were selected for their efficacy in inhibiting toxin metalloproteinase activity, with CPRGQPIIQDV demonstrating the most potent effect. CPRGQPIIQDV mainly presented beta-sheet and random coil structures, and significantly reduced the cytotoxicity and pro-inflammatory effects of NnNV on RAW264.7 cells. This study established a method for identifying metalloproteinase substrate sequences using crude jellyfish venom and provided an initial design and screening of peptide inhibitors, offering new insights into the management of jellyfish stings. |
| WOS关键词 | PHOSPHINIC PEPTIDES ; LIBRARIES ; ALPHA |
| 资助项目 | National Natural Science Foundation of China[42306143] ; China Postdoctoral Science Foundation[2023M733532] ; Doctoral Start-up Fund of Liaoning Normal University[2025BSL001] |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:001603535600001 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203734]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yu, Huahua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266000, Peoples R China 2.Liaoning Normal Univ, Sch Life Sci, Dalian 116081, Peoples R China 3.Liaoning Normal Univ, Liaoning Prov Key Lab Biotechnol & Drug Discovery, Dalian 116081, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Chunlin,Wang, Zhanhua,Wang, Wenjie,et al. Targeting marine jellyfish toxins: development of metalloproteinase inhibitors through a specific method for protease substrate identification[J]. ARCHIVES OF TOXICOLOGY,2025:18. |
| APA | Yu, Chunlin.,Wang, Zhanhua.,Wang, Wenjie.,Li, Rongfeng.,Xing, Ronge.,...&Yu, Huahua.(2025).Targeting marine jellyfish toxins: development of metalloproteinase inhibitors through a specific method for protease substrate identification.ARCHIVES OF TOXICOLOGY,18. |
| MLA | Yu, Chunlin,et al."Targeting marine jellyfish toxins: development of metalloproteinase inhibitors through a specific method for protease substrate identification".ARCHIVES OF TOXICOLOGY (2025):18. |
入库方式： OAI收割
来源：海洋研究所
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