Non-proteinogenic β-alanine metabolism in Halomonas sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules
文献类型：期刊论文
| 作者 | Guo, Shuju2,3; Xu, Xiuli4; Shuai, Hui1,5; Song, Fuhang6; Zhang, Guoliang7; Ma, Linlin8; Yang, Na2,3 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2025-11-18 |
| 页码 | 17 |
| 关键词 | Deep-sea ferromanganese nodules
Mn reduction
|
| ISSN号 | 0099-2240 |
| DOI | 10.1128/aem.02164-25 |
| 通讯作者 | Yang, Na(yangna@qdio.ac.cn) |
| 英文摘要 | Deep-sea ferromanganese nodules are valuable materials for investigating microorganism-mediated geochemical cycling of manganese. While many microbial metabolic pathways are closely associated with manganese reduction, few studies have examined the role of amino acid metabolism in the release of Mn(II) from these nodules. Here, we explored the impact of non-proteinogenic beta-alanine metabolism on manganese reduction. Halomonas sp. MNB13 is an indigenous bacterium isolated from a ferromanganese nodule that can reduce Mn oxides and decrease the pH of the environment during Mn(II) release. Comparative transcriptomic analysis unveiled that exposure to MnO2 significantly upregulated beta-alanine metabolism, leading to a reduced intracellular concentration of beta-alanine. Exogenous beta-alanine decreased the proportion of Mn(IV) without changing the type of Mn residues by promoting MNB13 growth and reshaping the profile of secreted organic acids. beta-Alanine elevated energy metabolism in MNB13 and increased catalase activity, mitigating reactive oxygen species generated during Mn(II) release and thereby enhancing bacterial growth. Among the secreted organic acids, levulinate and pantothenate showed altered abundance and were confirmed to promote Mn(II) release following beta-alanine supplementation. This study suggests that environmental beta-alanine modulates Mn(IV) reduction by promoting bacterial growth and organic acid secretion, offering novel insights into bacteria-mediated Mn(II) release from deep-sea ferromanganese nodules.IMPORTANCEMicroorganisms are believed to play a role in the biotic processes of deep-sea ferromanganese nodule formation and biosolubilization. Although most studies have linked microbial metabolism to manganese reduction, the role of microbial amino acid metabolism in the release of Mn(II) from ferromanganese nodules into seawater remains underexplored. beta-Alanine is a naturally occurring non-proteinogenic beta-amino acid widely distributed in the marine environment. However, its role in Mn(IV) reduction is unclear. This study demonstrated that Mn oxides upregulate beta-alanine metabolism in Halomonas sp. MNB13 and external beta-alanine significantly enhances Mn(II) release from Mn oxides. We showed that ferromanganese nodules induce beta-alanine metabolism and lead to the release of pantothenate and levulinate, decreasing the culture pH, reducing Mn(IV) to Mn(II), and resulting in Mn(II) release. These findings provide new insights into bacterial non-proteinogenic amino acid metabolism and its role in facilitating the Mn(II) release from ferromanganese nodules in deep-sea environments. |
| WOS关键词 | MICROBIAL MANGANESE ; OXIDE ; DISSOLUTION ; CATABOLISM ; MINERALS ; METALS |
| 资助项目 | Shandong Provincial Natural Science Foundation[ZR2025MS504] ; Posdoctoral Innovation Program of Shandong Province[SDCX-ZG-202303096] ; Qingdao New Energy Shandong Laboratory Open Project[QNESL OP202311] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001616416100001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203767]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yang, Na |
| 作者单位 | 1.Rizhao Polytech, Dept Marine Technol, Rizhao, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.China Univ Geosci, Sch Ocean Sci, Key Lab Marine Mineral Resources & Polar Geol, Minist Educ China, Beijing, Peoples R China 5.Lunan Pharmaceut Grp Co Ltd, State Key Lab Integrat & Innovat Class Formula & M, Linyi, Peoples R China 6.Beijing Technol & Business Univ, Sch Light Ind, Beijing, Peoples R China 7.Sun Yat sen Univ, Sch Marine Sci, Zhuhai, Peoples R China 8.Griffith Univ, Inst Biomed & Glyc, Sch Environm & Sci, Brisbane, Australia |
| 推荐引用方式 GB/T 7714 | Guo, Shuju,Xu, Xiuli,Shuai, Hui,et al. Non-proteinogenic β-alanine metabolism in Halomonas sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2025:17. |
| APA | Guo, Shuju.,Xu, Xiuli.,Shuai, Hui.,Song, Fuhang.,Zhang, Guoliang.,...&Yang, Na.(2025).Non-proteinogenic β-alanine metabolism in Halomonas sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,17. |
| MLA | Guo, Shuju,et al."Non-proteinogenic β-alanine metabolism in Halomonas sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules".APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2025):17. |
入库方式： OAI收割
来源：海洋研究所
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