A New Ribo-Type of Wangodinium sinense from Germination of Resting Cysts Isolated from Ballast Tank Sediments of Incoming Ships to China
文献类型：期刊论文
| 作者 | Tao, Zhe5,7; Yue, Caixia4,5,7; Liu, Yuyang3; Shi, Shuo5,7; Li, Ruoxi5,7; Chai, Zhaoyang1,5; Deng, Yunyan1,5; Shang, Lixia1,5; Hu, Zhangxi2; Gu, Haifeng6 |
| 刊名 | JOURNAL OF MARINE SCIENCE AND ENGINEERING
 |
| 出版日期 | 2025-05-12 |
| 卷号 | 13期号:5页码:15 |
| 关键词 | ballast tank sediments
dinoflagellate resting cysts
germination
ribo-type
|
| DOI | 10.3390/jmse13050942 |
| 通讯作者 | Li, Fengting(lifengting@qdio.ac.cn) ; Tang, Yingzhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | In recent decades, ships' ballast water and associated sediments have been recognized globally as significant vectors for the dissemination of non-indigenous species, which has attracted extensive attention due to its ecological and economic impacts. The characteristics of production of resting cysts in the dinoflagellate life cycle further increases the risk of biological invasions through ballast tank sediments. Despite extensive research which has characterized the species diversity of dinoflagellate cysts within ballast tank sediments, the possibility and importance of invasions caused by different ribosomal types of the same species have been paid little attention. In this study, two cultures of dinoflagellates were established through cyst germination from the ballast tank sediments collected from two ships ("THETIS" and "WARIYANAREE") arriving at the Jiangyin Port (China) and identified as Wangodinium sinense Z. Luo, Zhangxi Hu, Yingzhong Tang and H.F. Gu by comprehensive phylogenetic analysis of rDNA sequences (including LSU, SSU, and ITS1-5.8S-ITS2). Despite the rDNA sequences of the isolates showing a generally high similarity to reference sequences, the LSU D1-D6 sequences contained up to 11 stable single nucleotide polymorphisms (SNPs), while SSU and ITS1-5.8S-ITS2 sequences exhibited up to five and two divergence sites, respectively. Moreover, phylogenetic analyses based on partial LSU and SSU rDNA sequences further indicated that strains germinated from ships' ballast tank sediments formed a strongly supported sister clade to the strains previously isolated from Chinese and Korean waters, representing a novel ribo-type distinct from Chinese and Korean strains. Detailed morphological observations using light microscopy (LM) and scanning electron microscopy (SEM) did not find differences between our isolates and the holotype of the species in key diagnostic characteristics including the position and shape of the nucleus and chloroplasts, as well as the ASC structure, which suggested that no significant morphological divergence has occurred among these ribo-types. Acute toxicity exposure assays indicated that this ribo-type of W. sinense posed no lethal effect on rotifers at concentrations <= 104 cells/mL, yet it remains necessary to maintain vigilance regarding the potential risk of algal blooms resulting from higher cell density or environmental changes in the invaded ecosystems. This study reports the first successful germination of W. sinense cysts from ballast tank sediments, indicating that its cysts may be widely transferred through ballast tank sediments, and presents a potential risk of bio-invasions of new genotypes of species to a region where other genotypes of the same species have been present as indigenous species. |
| WOS关键词 | HARMFUL ALGAL BLOOMS ; DINOFLAGELLATE CYSTS ; GONYAULAX-TAMARENSIS ; WATER ; PHYTOPLANKTON ; ALEXANDRIUM ; TRANSPORT ; DINOPHYCEAE ; RELEVANCE ; IDENTIFICATION |
| 资助项目 | Key Research Infrastructures in the CAS Field Stations of the Chinese Academy of Science ; National Natural Science Foundation of China[42376138] ; [KFJ-SWYW047] |
| WOS研究方向 | Engineering ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001497726500001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202103]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Li, Fengting; Tang, Yingzhong |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 2.Guangdong Ocean Univ, Coll Fisheries, Dept Aquaculture, Zhanjiang 524088, Peoples R China 3.Shandong Univ Sci & Technol, Coll Safety & Environm Engn, Qingdao 266590, Peoples R China 4.Anim Plant & Food Inspect Ctr Nanjing Customs, Nanjing 210000, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 6.Minist Nat Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Zhe,Yue, Caixia,Liu, Yuyang,et al. A New Ribo-Type of Wangodinium sinense from Germination of Resting Cysts Isolated from Ballast Tank Sediments of Incoming Ships to China[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2025,13(5):15. |
| APA | Tao, Zhe.,Yue, Caixia.,Liu, Yuyang.,Shi, Shuo.,Li, Ruoxi.,...&Tang, Yingzhong.(2025).A New Ribo-Type of Wangodinium sinense from Germination of Resting Cysts Isolated from Ballast Tank Sediments of Incoming Ships to China.JOURNAL OF MARINE SCIENCE AND ENGINEERING,13(5),15. |
| MLA | Tao, Zhe,et al."A New Ribo-Type of Wangodinium sinense from Germination of Resting Cysts Isolated from Ballast Tank Sediments of Incoming Ships to China".JOURNAL OF MARINE SCIENCE AND ENGINEERING 13.5(2025):15. |
入库方式： OAI收割
来源：海洋研究所
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