The parasitic dinoflagellate Hematodinium perezi suppresses the innate immunity of Portunus trituberculatus via exosomal miRNAs
文献类型：期刊论文
| 作者 | Zhang, Ju2,3; Small, Hamish J.4; Li, Meng1,2,3; Huang, Qian2,3; Hu, Lijun2,3; Xue, Qi2,3; Wang, Jiayi2,3; Li, Caiwen1,2,3 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2025-10-15 |
| 卷号 | 609页码:14 |
| 关键词 | Hematodinium Exosomal miRNAs Immune suppression Innate immunity Portunus trituberculatus |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2025.742827 |
| 通讯作者 | Li, Meng(limeng@qdio.ac.cn) ; Li, Caiwen(cwli@qdio.ac.cn) |
| 英文摘要 | The parasitic dinoflagellate Hematodinium infects a wide range of crustaceans worldwide and causes epidemics, resulting in significant economic loss. It has become an emerging pathogenic threat to the sustainable development of marine crustacean aquaculture. However, there is still a lack of research on the interaction mechanisms between Hematodinium and its host, which significantly hinders the development of effective prevention and control strategies for this pathogen. Here, we used the parasitic dinoflagellate Hematodinium perezi and its crab host Portunus trituberculatus as an experimental model to explore how Hematodinium modulates its host's immune response to enable survival. We discovered that H. perezi was able to release exosomes, which were observed to be localized in the perinuclear region of hemocytes during parasitic infection. Two novel miRNAs (miRNA-PC-5p-81654-103 and miRNA-PC-5p-20520-538) involved in regulating the Toll-like receptor (TLR) signaling pathway were identified within exosomes of H. perezi. The miRNAs inhibited the expression of tlr4 and myeloid differentiation primary response 88 (MyD88)-mediated signal transduction pathway and resulted in subsequent suppression of the TLR signaling pathway in P. trituberculatus. Another miRNA (bmo-mir-6497-p5-1SS4AG) was found to modulate the host's mTOR-RhoA pathway. In conclusion, the H. perezi exosomal miRNAsmediated immunoregulatory processes significantly attenuated the crustacean host's immune capacity, and facilitated the survival and proliferation of the parasitic dinoflagellate within the host crustacean. This novel finding provides new insight into the host-parasite interaction. |
| WOS关键词 | LOBSTER NEPHROPS-NORVEGICUS ; MOLECULAR CHARACTERIZATION ; EXTRACELLULAR VESICLES ; GENE-EXPRESSION ; SWIMMING CRAB ; BLUE-CRAB ; SEASONAL PREVALENCE ; DEVELOPMENTAL CYCLE ; NECORA-PUBER ; WEST-COAST |
| 资助项目 | National Natural Science Foundation of China (NNSFC)[42006121] ; NSFC-Shandong Joint-funding Program[U1906214] ; Youth project of Natural Science Foundation of Shandong Province[ZR2020QD105] ; Postdoctoral Innovation Talents Support Program of Shandong[SDBX2023029] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001509821400001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202301]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Li, Meng; Li, Caiwen |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.William & Mary, Virginia Inst Marine Sci, Ecosyst Hlth Sect, Williamsburg 23062, VA USA |
| 推荐引用方式 GB/T 7714 | Zhang, Ju,Small, Hamish J.,Li, Meng,et al. The parasitic dinoflagellate Hematodinium perezi suppresses the innate immunity of Portunus trituberculatus via exosomal miRNAs[J]. AQUACULTURE,2025,609:14. |
| APA | Zhang, Ju.,Small, Hamish J..,Li, Meng.,Huang, Qian.,Hu, Lijun.,...&Li, Caiwen.(2025).The parasitic dinoflagellate Hematodinium perezi suppresses the innate immunity of Portunus trituberculatus via exosomal miRNAs.AQUACULTURE,609,14. |
| MLA | Zhang, Ju,et al."The parasitic dinoflagellate Hematodinium perezi suppresses the innate immunity of Portunus trituberculatus via exosomal miRNAs".AQUACULTURE 609(2025):14. |
入库方式： OAI收割
来源：海洋研究所
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