Differential Expression of Hsp100 Gene in Scrippsiella acuminata: Potential Involvement in Life Cycle Transition and Dormancy Maintenance
文献类型：期刊论文
| 作者 | Li, Fengting1,4,5; Shang, Lixia1,4,5; Zou, Hanying1,2; Sun, Chengxing1,2; Hu, Zhangxi3; Tang, Ying Zhong1,4,5; Deng, Yunyan1,4,5 |
| 刊名 | DIVERSITY-BASEL
 |
| 出版日期 | 2025-07-26 |
| 卷号 | 17期号:8页码:13 |
| 关键词 | dinoflagellate dormancy maintenance harmful algal blooms heat shock protein 100 (Hsp100) parallel reaction monitoring (PRM) resting cyst |
| DOI | 10.3390/d17080519 |
| 通讯作者 | Deng, Yunyan(yunyandeng@qdio.ac.cn) |
| 英文摘要 | Protein degradation plays a fundamental role in maintaining protein homeostasis and ensures proper cellular function by regulating protein quality and quantity. Heat shock protein 100 (Hsp100), found in bacteria, plants, and fungi, is a unique chaperone family responsible for rescuing misfolded proteins from aggregated states in an ATP-dependent manner. To date, they are primarily known to mediate heat stress adaptation and enhance cellular survival under extreme conditions in higher plants and algae. Resting cyst formation in dinoflagellates is widely recognized as a response to adverse conditions, which offers an adaptive advantage to endure harsh environmental extremes that are unsuitable for vegetative cell growth and survival. In this study, based on a full-length cDNA sequence, we characterized an Hsp100 gene (SaHsp100) from the cosmopolitan bloom-forming dinoflagellate Scrippsiella acuminata, aiming to examine its life stage-specific expression patterns and preliminarily explore its potential functions. The qPCR results revealed that Hsp100 transcript levels were significantly elevated in newly formed resting cysts compared to vegetative cells and continued to increase during storage under simulated marine sediment conditions (darkness, low temperature, and anoxia). Parallel reaction monitoring (PRM)-based quantification further confirmed that Hsp100 protein levels were significantly higher in resting cysts than in vegetative cells and increased after three months of storage. These findings collectively highlighted the fundamental role of Hsp100 in the alteration of the life cycle and dormancy maintenance of S. acuminata, likely by enhancing stress adaptation and promoting cell survival through participation in proteostasis maintenance, particularly under natural sediment-like conditions that trigger severe abiotic stress. Our work deepens the current understanding of Hsp family members in dinoflagellates, paving the way for future investigations into their ecological relevance within this ecologically significant group. |
| WOS关键词 | HEAT-SHOCK PROTEINS ; STRESS ; TIME ; EVOLUTIONARY |
| 资助项目 | the National Natural Science Foundation of China[42406214] ; the National Natural Science Foundation of China[42206215] ; National Natural Science Foundation of China |
| WOS研究方向 | Biodiversity & Conservation ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001558560100001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203230]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Deng, Yunyan |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Fengting,Shang, Lixia,Zou, Hanying,et al. Differential Expression of Hsp100 Gene in Scrippsiella acuminata: Potential Involvement in Life Cycle Transition and Dormancy Maintenance[J]. DIVERSITY-BASEL,2025,17(8):13. |
| APA | Li, Fengting.,Shang, Lixia.,Zou, Hanying.,Sun, Chengxing.,Hu, Zhangxi.,...&Deng, Yunyan.(2025).Differential Expression of Hsp100 Gene in Scrippsiella acuminata: Potential Involvement in Life Cycle Transition and Dormancy Maintenance.DIVERSITY-BASEL,17(8),13. |
| MLA | Li, Fengting,et al."Differential Expression of Hsp100 Gene in Scrippsiella acuminata: Potential Involvement in Life Cycle Transition and Dormancy Maintenance".DIVERSITY-BASEL 17.8(2025):13. |
入库方式： OAI收割
来源：海洋研究所
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