Effects of estradiol on lipid metabolism, gamete development, and maturation during reproduction in sea cucumber Apostichopus japonicus and its potential signaling-regulated network
文献类型：期刊论文
| 作者 | Zhang, Yanni1,3,4,5; Eeckhaut, Igor2; Zhang, Libin1,3,4,5 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2026-01-15 |
| 卷号 | 612页码:13 |
| 关键词 | Apostichopus japonicus Estradiol Lipid metabolism Gamete development and maturation Signaling regulation |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2025.743116 |
| 通讯作者 | Zhang, Libin(zhanglibin@qdio.ac.cn) |
| 英文摘要 | Sea cucumber (Apostichopus japonicus) is an important aquacultured species due to its economic and medicinal values. Reproduction is a key process in sea cucumber aquaculture and gonadal development is the basis of reproduction. Estrogen, as one of the important reproductive endocrine substances, is involved in many physiological processes regulating reproduction. However, whether estrogen can induce gonadal development of A. japonicus and its potential mechanism have not been deeply addressed. Here, we exposed sea cucumbers to 0.1 mu g / L, 1 mu g / L, 10 mu g / L E2 for 30 days, and found that the male and female gonadosomatic index increased significantly in 10 mu g / L and 1 mu g / L E2, respectively. In addition, E2 treatment significantly promoted the expression level of Vg mRNA in ovaries with the best promotion effect with a concentration of 1 mu g / L. Then, liquid chromatography-mass spectrometry and RNA sequencing were used to identify differentially accumulated metabolites and differentially expressed genes after E2 (1 mu g / L) interference. Combined analysis of transcription and metabolism showed that E2 activated male fatty acid synthesis-related genes promoting lipid accumulation. Compared to males, lipid production in female was limited, while oxidation was increased, providing more energy for ovarian development. Finally, we verified the potential signaling pathways which mediate the E2 effect in A. japonicus gonadal development. The qRT-PCR analysis showed that E2 likely regulate lipid metabolism and oocyte maturation in female sea cucumber by down-regulating some pathways, including cAMP, FOXO and mTOR. In conclusion, our analysis showed that (i) exogenous E2 regulated the A. japonicus gonadal development through the non-genomic effect of membrane receptors, (ii) the effect of E2 was dose-dependent and (iii) sex-specific. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; FATTY-ACID SYNTHESIS ; MEIOTIC ARREST ; ESTROGEN ; MAINTENANCE ; GROWTH ; CELL ; STRINGTIE ; STEROIDS ; PROGRESS |
| 资助项目 | National Key Research and Development Program of China[2022YFE0140900] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001565020900001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203281]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Zhang, Libin |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Univ Mons, Biol Marine Organisms & Biomimetism Lab, B-7000 Mons, Belgium 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266000, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yanni,Eeckhaut, Igor,Zhang, Libin. Effects of estradiol on lipid metabolism, gamete development, and maturation during reproduction in sea cucumber Apostichopus japonicus and its potential signaling-regulated network[J]. AQUACULTURE,2026,612:13. |
| APA | Zhang, Yanni,Eeckhaut, Igor,&Zhang, Libin.(2026).Effects of estradiol on lipid metabolism, gamete development, and maturation during reproduction in sea cucumber Apostichopus japonicus and its potential signaling-regulated network.AQUACULTURE,612,13. |
| MLA | Zhang, Yanni,et al."Effects of estradiol on lipid metabolism, gamete development, and maturation during reproduction in sea cucumber Apostichopus japonicus and its potential signaling-regulated network".AQUACULTURE 612(2026):13. |
入库方式： OAI收割
来源：海洋研究所
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