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In situ semi-quantitative imaging of intracellular metabolic interaction by confocal Raman microscopy

文献类型：期刊论文

作者He, Wanying10; Wang, Minxiao2,3,5,6,7; Zhong, Zhaoshan5,6,7; Chen, Hao5,6,7; Xi, Shichuan10; Zhang, Huan2,3,5,6,7; Li, Mengna2,3,5,6,7; Sun, Wenhao2,3,8; Zhang, Yan8; Wang, Yun9
刊名ISCIENCE
出版日期2025-10-17
卷号28期号:10页码:12
DOI10.1016/j.isci.2025.113558
通讯作者Li, Chaolun(lcl@qdio.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn)
英文摘要Non-destructive subcellular metabolite quantification can reveal critical insights into biological interactions (e.g., endosymbiont-host crosstalk). Therefore, we developed a multivariate semi-quantitative imaging method using internal standardization to resolve simultaneous subcellular distributions of multiple metabolites, leveraging confocal Raman microscopy's (CRM's) high spatial resolution. The method was applied to the endosymbiotic mussel Gigantidas platifrons, whose symbiotic interaction mechanism has not been elucidated because symbionts cannot be cultivated. The results showed that the aggregated distribution of distinct phenotypes of symbiont strains was characterized by different glycogen abundances, indicating niche-driven metabolic strategies. Our data may provide direct evidence suggesting that symbionts supply intermediates to the host for cholesterol synthesis, potentially via vesicular trafficking. This work demonstrates CRM's capacity for comparative, spatially resolved metabolite quantification across cellular compartments. While semi-quantitative, CRM emerges as a powerful non-invasive tool for probing metabolic network dynamics and compartmentalization in challenging biological systems where traditional methods are limited.
WOS关键词VIBRATIONAL-SPECTRA ; MASS-SPECTROMETRY ; SPECTROSCOPY ; IDENTIFICATION ; METHANOTROPH ; INFORMATION ; METHANE
资助项目National Natural Science Foundation of China[42327805] ; National Natural Science Foundation of China[42221005] ; National Natural Science Foundation of China[42030407] ; National Natural Science Foundation of China[42506179] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology[2022QNLM030004-3] ; China Postdoctoral Science Foundation[2024M751269] ; Shandong Provincial Natural Science Foundation[ZR2024QD074]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001584538600003
出版者CELL PRESS
源URL[http://ir.qdio.ac.cn/handle/337002/203533]  
专题海洋研究所_海洋生态与环境科学重点实验室
通讯作者Li, Chaolun; Zhang, Xin
作者单位1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Key Lab Ocean Observat & Forecasting, CAS Key Lab Marine Geol & Environm, Ctr Ocean Mega Sci,Inst Oceanol, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China
7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266061, Peoples R China
8.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China
9.Univ OSCAR, Oxford Suzhou Ctr Adv Res, Suzhou 215123, Peoples R China
10.Laoshan Lab, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 He, Wanying,Wang, Minxiao,Zhong, Zhaoshan,et al. In situ semi-quantitative imaging of intracellular metabolic interaction by confocal Raman microscopy[J]. ISCIENCE,2025,28(10):12.
APA He, Wanying.,Wang, Minxiao.,Zhong, Zhaoshan.,Chen, Hao.,Xi, Shichuan.,...&Zhang, Xin.(2025).In situ semi-quantitative imaging of intracellular metabolic interaction by confocal Raman microscopy.ISCIENCE,28(10),12.
MLA He, Wanying,et al."In situ semi-quantitative imaging of intracellular metabolic interaction by confocal Raman microscopy".ISCIENCE 28.10(2025):12.

入库方式： OAI收割

来源：海洋研究所

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