A newly developed Germlings Harvester (GEHA) in combination with metabarcoding analysis detected numerous plankton species, particularly HABs-causing species, from in-situ germination of resting stage cells
文献类型：期刊论文
| 作者 | Shi, Shuo2,4; Tao, Zhe2,4,6; Yang, Wanli2,4; Li, Fengting2,3; Wei, Ben2,5; Yue, Caixia1,2; Pan, Suzhi2; Deng, Yunyan2,3; Shang, Lixia2,3; Chai, Zhaoyang2,3 |
| 刊名 | HARMFUL ALGAE
 |
| 出版日期 | 2025-12-01 |
| 卷号 | 150页码:17 |
| 关键词 | In-situ monitoring of germination Resting-stage cell (RSC) Resting cyst Germlings Harvester (GEHA) Plankton Dinoflagellate |
| ISSN号 | 1568-9883 |
| DOI | 10.1016/j.hal.2025.103002 |
| 通讯作者 | Chai, Zhaoyang(zhaoyangchai@qdio.ac.cn) ; Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | Germination of resting-stage cells (RSCs), including the resting cysts of dinoflagellates and raphidophytes and resting spores of diatoms, of plankton influence community composition, species abundance, and population dynamics, particularly the initiation of harmful algal blooms (HABs), in aquatic ecosystem. While the vital role played by resting cysts in seeding HABs has been well known in the HABs research community, in-situ detection of RSCs germination in the field sediment, however, remains largely unexplored due to the lack of feasible facility and accurate identification method, and many important groups of plankton have even not been systematically investigated for their life histories. We therefore developed a facility, named Germlings Harvester (GEHA), for the in-situ detection of the species diversity of plankton RSCs, particularly those with vital importance, that germinated from the natural marine sediment. Using GEHA in combination with eukaryote-targeted metabarcoding analysis and other approaches in Jiaozhou Bay, China, we identified 220 planktonic species germinated in situ, including 51 species of Dinoflagellata, 54 Bacillariophyta, 16 Ochrophyta, 18 Chlorophyta, 9 Haptophyta, 31 Ciliophora, 2 Euglenozoa, 3 Amoebozoa, and 36 others. Among these species, 38 are HABscausing or toxic (notably Akashiwo sanguinea, Azadinium polongum, Karlodinium veneficum, Margalefidinium fulvescens, Prorocentrum donghaiense, Pseudonitzschia spp., Heterosigma akashiwo, Aureococcus anophagefferens, and Phaeocystis globosa, all being observed for their in-situ germinations of RSCs in the field for the first time), one ciliate reported to form "red tides", and five parasites reported highly harmful to their hosts. It is also noteworthy that 128 of the 220 species were observed as novel RSC producers, and four are freshwater species. In addition, eight germinated species isolated from the germlings with GEHA were identified via establishing clonal cultures and sequencing or single-cell sequencing (e.g., A. sanguinea). Our results demonstrated not only the robustness of the GEHA-based approach in the field investigation of plankton population dynamics and particularly HABs ecology from the facet of germination dynamics of RSCs (cysts, spores and akinetes), but also their vital importance in understanding the general ecology of plankton and aquatic ecosystems and in the monitoring and forecasting of HABs. |
| WOS关键词 | HARMFUL ALGAL BLOOMS ; COCHLODINIUM-FULVESCENS GYMNODINIALES ; ALEXANDRIUM-FUNDYENSE ; DINOFLAGELLATE CYSTS ; SAMPLING DEVICE ; ONAGAWA BAY ; DINOPHYCEAE ; DIVERSITY ; GULF ; PHYTOPLANKTON |
| 资助项目 | Key Research In-frastructures in the CAS Field Stations of the Chinese Academy of Sci-ence[KFJ-SWYW047] ; Science and Technology Innovation Project of Laoshan Laboratory[LSKJ202203700] ; National Science Foundation of China[U23A203] ; National Science Foundation of China[42406210] ; Shandong Provincial Natural Science Foundation[ZR2024MD109] |
| WOS研究方向 | Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001602500200001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203686]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chai, Zhaoyang; Tang, Ying Zhong |
| 作者单位 | 1.Anim Plant & Food Inspect Ctr Nanjing Customs, Nanjing 210000, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Harbin Engn Univ, Qingdao Innovat & Dev Ctr, Qingdao 266000, Peoples R China 6.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Shuo,Tao, Zhe,Yang, Wanli,et al. A newly developed Germlings Harvester (GEHA) in combination with metabarcoding analysis detected numerous plankton species, particularly HABs-causing species, from in-situ germination of resting stage cells[J]. HARMFUL ALGAE,2025,150:17. |
| APA | Shi, Shuo.,Tao, Zhe.,Yang, Wanli.,Li, Fengting.,Wei, Ben.,...&Tang, Ying Zhong.(2025).A newly developed Germlings Harvester (GEHA) in combination with metabarcoding analysis detected numerous plankton species, particularly HABs-causing species, from in-situ germination of resting stage cells.HARMFUL ALGAE,150,17. |
| MLA | Shi, Shuo,et al."A newly developed Germlings Harvester (GEHA) in combination with metabarcoding analysis detected numerous plankton species, particularly HABs-causing species, from in-situ germination of resting stage cells".HARMFUL ALGAE 150(2025):17. |
入库方式： OAI收割
来源：海洋研究所
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