Comparison of Environmental DNA Metabarcoding and Underwater Visual Census for Assessing Macrobenthic Diversity
文献类型：期刊论文
| 作者 | Zhan, Zifeng1,2,5; Huo, Weiwei2,5; Xie, Shangwei4; Chen, Wandong4; Liu, Xinming3; Xu, Kuidong1,2; Lei, Yanli1,2 |
| 刊名 | BIOLOGY-BASEL
 |
| 出版日期 | 2025-07-06 |
| 卷号 | 14期号:7页码:20 |
| 关键词 | eDNA species detection efficiency marine protected area subtidal zone biodiversity assessment |
| DOI | 10.3390/biology14070821 |
| 通讯作者 | Lei, Yanli(leiyanli@qdio.ac.cn) |
| 英文摘要 | The rapid advancement of environmental DNA (eDNA) technology has transformed ecological research, particularly in aquatic ecosystems. However, the optimal sampling matrix (e.g., water or sediment) and the potential for eDNA to replace or complement traditional underwater visual census (UVC) remain unclear. Here, we integrate water eDNA, sediment eDNA, and UVC approaches to systematically compare the diversity of benthic macrofauna in the subtidal zones of the Nanji Islands, China. Our results show that sediment eDNA samples exhibited the highest species richness, while UVC had the lowest. Each method revealed distinct species profiles, with relatively few shared taxa at the order level and below. Environmental eDNA showed significant advantages in detecting key phyla such as Annelida and Arthropoda. In contrast, traditional UVC was crucial for identifying certain taxa, such as Bryozoa, which were undetectable by eDNA methods. The low overlap in species detected by these methods underscores their complementary nature, highlighting the necessity of integrating multiple approaches to achieve a more comprehensive and accurate biodiversity assessment. Future research should focus on refining eDNA techniques, such as developing more universal primers, to further enhance their applicability in biodiversity monitoring. |
| 资助项目 | National Key R&D Program of China ; National Natural Science Foundation of China[42176128] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Science and Technology Program of Nanji Islands National Marine Nature Reserve Administration[2022YFC2803800] ; Science and Technology Program of Nanji Islands National Marine Nature Reserve Administration[ZJYY-PYCG-2024092001] ; Science and Technology Program of Nanji Islands National Marine Nature Reserve Administration[JJZB-PYCG-2021112901] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001535553600001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202689]  |
| 专题 | 海洋研究所_海洋生物分类与系统演化实验室 |
| 通讯作者 | Lei, Yanli |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China 3.Guangxi Univ Chinese Med, Inst Marine Drugs, Nanning 530200, Peoples R China 4.Nanji Isl Natl Marine Nat Reserve Adm, Wenzhou 325400, Peoples R China 5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhan, Zifeng,Huo, Weiwei,Xie, Shangwei,et al. Comparison of Environmental DNA Metabarcoding and Underwater Visual Census for Assessing Macrobenthic Diversity[J]. BIOLOGY-BASEL,2025,14(7):20. |
| APA | Zhan, Zifeng.,Huo, Weiwei.,Xie, Shangwei.,Chen, Wandong.,Liu, Xinming.,...&Lei, Yanli.(2025).Comparison of Environmental DNA Metabarcoding and Underwater Visual Census for Assessing Macrobenthic Diversity.BIOLOGY-BASEL,14(7),20. |
| MLA | Zhan, Zifeng,et al."Comparison of Environmental DNA Metabarcoding and Underwater Visual Census for Assessing Macrobenthic Diversity".BIOLOGY-BASEL 14.7(2025):20. |
入库方式： OAI收割
来源：海洋研究所
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