Integration of exonuclease III-assisted target recovery and enzymatic catalysis in OPECT biosensor for ultrasensitive detection of bacterial quorum sensing signal molecules
文献类型：期刊论文
| 作者 | Liu, Zhiru1; Chi, Jingtian1; Wang, Jinquan4; Xiao, Long4; Wang, Shuai1; Zheng, Zhou1,3; Ma, Fubin2; Ju, Peng1 |
| 刊名 | MICROCHEMICAL JOURNAL
 |
| 出版日期 | 2025-07-01 |
| 卷号 | 214页码:9 |
| 关键词 | OPECT Photoelectrochemistry MOFs Heterojunction Quorum sensing signal molecules |
| ISSN号 | 0026-265X |
| DOI | 10.1016/j.microc.2025.113998 |
| 通讯作者 | Zheng, Zhou(zhengzhou@fio.org.cn) ; Ma, Fubin(mafubin@qdio.ac.cn) ; Ju, Peng(jupeng@fio.org.cn) |
| 英文摘要 | Quorum sensing signal molecules (3-O-C10-HL) regulate marine biofouling on artificial substrates, necessitating a quick detection method to track its onset and development. In this study, an innovative NH2-UiO-66/CdS gated organic photoelectrochemical transistor (OPECT) biosensor was contrived and deployed for the sensitive detection of 3-O-C10-HL, employing an exonuclease (Exo) III-facilitated enzymatic amplification strategy for the manipulation of cDNA. Notably, the NH2-UiO-66/CdS type II heterojunction was meticulously synthesized and demonstrated their proficiency in modulating the poly(3, 4-ethylenedioxythiophene): polystyrene sulfonate channel. The specific adsorption of 3-O-C10-HL to the self-screened aptamer elicited the release of cDNA, which in turn engaged in an Exo III-mediated enzymatic cycling process, culminating in the formation of magnetic beads/dsDNA3-Au nanoparticals@ascorbic acid-2-phosphate (AAP) complexes. The subsequent coalescence of these complexes with AAP resulted in the production of the electron donor ascorbic acid, thereby enhancing the photoconversion efficiency of NH2-UiO-66/CdS photoelectrode and modifying the channel current response. Remarkably, the fabricated device manifested a current amplification of 1150, a linear detection range spanning from 10-3 to 10-9 M, and an impressive detection limit of 0.967 pM. This study introduces an innovative methodology for the detection of 3-O-C10-HL, which paves new avenues for the effective surveillance of the onset and progression of marine biofouling. |
| WOS关键词 | HOMOSERINE LACTONE ; BIOFILM FORMATION |
| 资助项目 | National Natural Science Foundation of China[42476042] ; Wenhai Program of the S &T Fund of Shandong Province for Laoshan Laboratory ; Basic Scientific Fund for National Public Research Institutes of China ; Open Fund of Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention ; China Scholarship Council ; [42176242] ; [2021WHZZB2305] ; [2021Q10] ; [202203] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001499013200006 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/202199]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Zheng, Zhou; Ma, Fubin; Ju, Peng |
| 作者单位 | 1.Minist Nat Resources, Inst Oceanog 1, Marine Bioresource & Environm Res Ctr, Marine Nat Prod Res & Dev Key Lab Qingdao, 6 Xianxialing Rd, Qingdao 266061, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Adv Marine Mat, CAS Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 4.Ningbo Hangzhou Bay Bridge Dev Co LTD, Ningbo 315327, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Zhiru,Chi, Jingtian,Wang, Jinquan,et al. Integration of exonuclease III-assisted target recovery and enzymatic catalysis in OPECT biosensor for ultrasensitive detection of bacterial quorum sensing signal molecules[J]. MICROCHEMICAL JOURNAL,2025,214:9. |
| APA | Liu, Zhiru.,Chi, Jingtian.,Wang, Jinquan.,Xiao, Long.,Wang, Shuai.,...&Ju, Peng.(2025).Integration of exonuclease III-assisted target recovery and enzymatic catalysis in OPECT biosensor for ultrasensitive detection of bacterial quorum sensing signal molecules.MICROCHEMICAL JOURNAL,214,9. |
| MLA | Liu, Zhiru,et al."Integration of exonuclease III-assisted target recovery and enzymatic catalysis in OPECT biosensor for ultrasensitive detection of bacterial quorum sensing signal molecules".MICROCHEMICAL JOURNAL 214(2025):9. |
入库方式： OAI收割
来源：海洋研究所
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