Biosynthesis of CuS nanozymes for synergistic antibacterial applications: Biosynthetic parameters optimization and antimicrobial mechanisms elucidation
文献类型：期刊论文
| 作者 | Yang, Jing1,2; Zhai, Xiaofan1,5; Zhang, Shiqi1,2; Wang, Wenjie1,2; Ju, Peng4; Wang, Chenlu1,3; Duan, Jizhou1,5; Hou, Baorong1,5 |
| 刊名 | JOURNAL OF COLLOID AND INTERFACE SCIENCE
 |
| 出版日期 | 2025-12-15 |
| 卷号 | 700页码:13 |
| 关键词 | Sulfate-reducing bacteria Biosynthesis Nanozymes Antibacterials |
| ISSN号 | 0021-9797 |
| DOI | 10.1016/j.jcis.2025.138604 |
| 通讯作者 | Zhai, Xiaofan(zhaixf@qdio.ac.cn) |
| 英文摘要 | The imperative to address antibiotic-resistant bacterial infections necessitates the development of novel antibacterial materials and methodologies, with nanozymes exhibiting peroxidase-like activity emerging as a highly promising option. Here, sulfate-reducing bacteria served as bioreactors to biosynthesize copper sulfide nano-particles (Bio-CuS NPs) via dissimilatory sulfate reduction pathways. By optimizing cultivation parameters such as pH, Cu2+ concentration, and carbon source, we successfully produced Bio-CuS NPs with a reduced size, enhanced crystallinity, and superior peroxidase-like activity. Comparative studies with chemically synthesized copper sulfide (Abio-CuS NPs) revealed that Bio-CuS NPs were coated with biomolecules, possessed a greater specific surface area, exhibited abundant sulfur vacancies, and demonstrated higher peroxidase-like activity. Leveraging these properties, Bio-CuS NPs demonstrated a 99.99 % antibacterial efficacy against Escherichia coli and Staphylococcus aureus within 2 h at low H2O2 concentrations (0.8 mM). The antibacterial mechanism was attributed to the synergistic generation of hydroxyl radicals (center dot OH) and the release of Cu2+, which sequentially disrupted bacterial cell structure and ultimately led to metabolic dysfunction. In addition, the antibacterial Bio-CuS NPs exhibited outstanding biocompatibility, rendering them highly suitable for environmental applications. This study established a green biosynthesis platform for the production of nanozymes, highlighting the potential of bio-nanoparticles as sustainable antimicrobial agents with dual advantages of eco-friendly production and catalytic bactericidal efficacy. |
| WOS关键词 | NANOPARTICLES ; PERFORMANCE ; COMPOSITE |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB1210302] ; Guangxi Science and Technology Program[Guike AA23026007] ; National Natural Science Foundation of China[42376204] ; National Natural Science Foundation of China[42476042] ; Shandong Provincial Natural Science Foundation[ZR2022MD023] ; International Partnership Program of Chinese Academy of Sciences[058GJHZ2023058FN] ; Key R&D Program of Shandong Province, China[2022CXPT027] ; Key R&D Program of Shandong Province, China[2023CXPT008] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001548305700001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/203164]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Zhai, Xiaofan |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, State Key Lab Adv Marine Mat, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking LBM, Jinan 250353, Peoples R China 4.Minist Nat Resources, Inst Oceanog 1, Marine Bioresource & Environm Res Ctr, Marine Nat Prod Res & Dev Key Lab Qingdao, Qingdao 266061, Peoples R China 5.Guangxi Acad Sci, Inst Marine Corros Protect, Guangxi Key Lab Marine Environm Sci, Nanning 530007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jing,Zhai, Xiaofan,Zhang, Shiqi,et al. Biosynthesis of CuS nanozymes for synergistic antibacterial applications: Biosynthetic parameters optimization and antimicrobial mechanisms elucidation[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2025,700:13. |
| APA | Yang, Jing.,Zhai, Xiaofan.,Zhang, Shiqi.,Wang, Wenjie.,Ju, Peng.,...&Hou, Baorong.(2025).Biosynthesis of CuS nanozymes for synergistic antibacterial applications: Biosynthetic parameters optimization and antimicrobial mechanisms elucidation.JOURNAL OF COLLOID AND INTERFACE SCIENCE,700,13. |
| MLA | Yang, Jing,et al."Biosynthesis of CuS nanozymes for synergistic antibacterial applications: Biosynthetic parameters optimization and antimicrobial mechanisms elucidation".JOURNAL OF COLLOID AND INTERFACE SCIENCE 700(2025):13. |
入库方式： OAI收割
来源：海洋研究所
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