Size-dependent interactions between calciprotein particles and vascular endothelium
文献类型：期刊论文
| 作者 | Zhang, Zeping1,2; Wang, Xinyue1,2; Huang, Caihao1,3; Wang, Meixia4; Cui, Wei1,2; Hao, Liang5; Yang, Rui1,2; Wang, Hong-hui4; Zhang, Xing1,2 |
| 刊名 | MATERIALS TODAY BIO
 |
| 出版日期 | 2025-04-01 |
| 卷号 | 31页码:12 |
| 关键词 | Endothelium Calciprotein nanoparticles Chronic kidney disease Biomimetic synthesis |
| ISSN号 | 2590-0064 |
| DOI | 10.1016/j.mtbio.2025.101599 |
| 通讯作者 | Wang, Hong-hui(wanghonghui@hnu.edu.cn) ; Zhang, Xing(xingzhang@imr.ac.cn) |
| 英文摘要 | The underlying mechanisms governing the interactions between nanoparticles and vascular endothelial barrier remain largely unexplored, which is crucial for the optimal design of nanoparticles for clinical applications. In this study, the size-dependent interactions between calciprotein particles (CPPs) and endothelial cells (ECs) were investigated using a rat model of chronic kidney disease (CKD) induced by 5/6 nephrectomy. Two primary types of CPP1 were studied: small-sized CPP1 (S-CPP1, <50 nm) and larger CPP1 (L-CPP1, <100 nm), detected three and five weeks post-surgery, respectively. By adjusting the amounts of Ca2+, HPO42- and H2PO4- ions in Dulbecco's Modified Eagle Medium supplemented with 10 % (V/V) fetal bovine serum and 1 % (V/V) Pen-Strep solution, S-CPP1 (<50 nm) with an elliptical shape, L-CPP1 (50-100 nm), and secondary CPPs (CPP2, >100 nm) with a needle-like crystalline structure, resembling endogenous CPPs, were synthesized. The results showed that S-CPP1 significantly increased endothelial permeability at concentrations of 445 mu g/mL and 890 mu g/mL, thereby disrupting the integrity of the endothelial barrier formed by a confluent monolayer of ECs. Immunofluorescence analysis revealed that L-CPP1 was internalized by ECs via endocytosis, while S-CPP1 disrupted VE-cadherin junctions, leading to irregular cell morphology and widened intercellular gaps. These structural changes likely contribute to medial calcification as CPPs accumulate within the circulatory system. In conclusion, the findings underscore that the interaction between CPPs and the vascular endothelium is strongly size-dependent, with significant implications for vascular system health and the design of nanoparticle-based therapies. |
| 资助项目 | National Natural Science Foundation of China[52273278] ; Liaoning Provincial Science and Technology Plan Project[2022-YGJC-32] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001436871000001 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; Liaoning Provincial Science and Technology Plan Project |
| 源URL |  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Wang, Hong-hui; Zhang, Xing |
| 作者单位 | 1.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 2.Univ Sci & Technol China, Sch Mat Sci & Engn, Hefei 230026, Anhui, Peoples R China 3.Dalian Univ Technol, Sch Mat Sci & Engn, Dalian 116024, Liaoning, Peoples R China 4.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China 5.China Med Univ, Sch Forens Med, Shenyang 110026, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zeping,Wang, Xinyue,Huang, Caihao,et al. Size-dependent interactions between calciprotein particles and vascular endothelium[J]. MATERIALS TODAY BIO,2025,31:12. |
| APA | Zhang, Zeping.,Wang, Xinyue.,Huang, Caihao.,Wang, Meixia.,Cui, Wei.,...&Zhang, Xing.(2025).Size-dependent interactions between calciprotein particles and vascular endothelium.MATERIALS TODAY BIO,31,12. |
| MLA | Zhang, Zeping,et al."Size-dependent interactions between calciprotein particles and vascular endothelium".MATERIALS TODAY BIO 31(2025):12. |
入库方式： OAI收割
来源：金属研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。