Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation
文献类型：期刊论文
| 作者 | Li, Long-Fei1,2; Chan, Ben Chung-Lap1,2; Yue, Grace Gar-Lee1,2; Lau, Clara Bik-San1,2; Han, Quan-Bin4; Leung, Ping-Chung1,2; Liu, Ji-Kai5; Fung, Kwok-Pui1,2,3 |
| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2013-10-15 |
| 卷号 | 20期号:13页码:1196-1202 |
| 关键词 | Pistillarin 1-ribofuranosyl-s-triazin-2(1H)-one Rubinoboletus ballouii bioassay-guided fractionation peripheral blood mononuclear cells (PBMCs) |
| ISSN号 | 0944-7113 |
| 通讯作者 | Fung, KP (reprint author), Chinese Univ Hong Kong, Inst Chinese Med, E205,Sci Ctr East Block, Shatin, Hong Kong, Peoples R China.,jkliu@mail.kib.ac.cn ; kpfung@cuhk.edu.hk |
| 英文摘要 | Rubinoboletus ballouii is an edible mushroom wildly grown in Yunnan province, China. Up till now, little was known about the chemical and biological properties of this mushroom. The aim of this study was to investigate the immunomodulatory effects of the ethanolic extract of Rubinoboletus ballouii and its fractions on human peripheral blood mononuclear cells (PBMCs) using bioactivity-guided fractionation. The crude extract of the fruiting bodies of RB was fractionated by high-speed counter current chromatography (HSCCC). Twelve fractions were obtained and the third fraction (Fraction C) exerted the most potent anti-inflammatory activities in mitogen-activated PBMCs. Further fractionation of fraction C led to the isolation of two single compounds which were elucidated as 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin, respectively. The results showed that both 1-ribofuranosyl-s-triazin-2(1H)-one and pistillarin exhibited significant immunosuppressive effects on phytohemagglutinin (PHA)-stimulated human PBMCs by inhibiting [methyl-H-3]-thymidine uptake and inflammatory cytokines productions such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-10, interferon (IFN)-gamma and IL-1 beta. Besides, 1-ribofuranosyl-s-triazin-2(1H)-one was-firstly-found in-natural-resources, and pistillarin was also-isolated-from-the-family Boletaceae for the first time. They exhibited great potential in developing as anti-inflammatory reagents. (C) 2013 Elsevier GmbH. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 类目[WOS] | Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; 5-AZA-2'-DEOXYCYTIDINE ; ANTIOXIDANT ; ACTIVATION ; GENE ; TNF |
| 收录类别 | SCI |
| 资助信息 | C.C. Wu Cultural & Education Foundation Fund; The Open Fund of the State Key Laboratory of Phytochemistry and Plant Resources in West China [P2009-KF03] |
| 语种 | 英语 |
| WOS记录号 | WOS:000325445400008 |
| 公开日期 | 2013-11-12 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/17277]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 2.Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources West Ch, Shatin, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China 4.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Long-Fei,Chan, Ben Chung-Lap,Yue, Grace Gar-Lee,et al. Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation[J]. PHYTOMEDICINE,2013,20(13):1196-1202. |
| APA | Li, Long-Fei.,Chan, Ben Chung-Lap.,Yue, Grace Gar-Lee.,Lau, Clara Bik-San.,Han, Quan-Bin.,...&Fung, Kwok-Pui.(2013).Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation.PHYTOMEDICINE,20(13),1196-1202. |
| MLA | Li, Long-Fei,et al."Two immunosuppressive compounds from the mushroom Rubinoboletus ballouii using human peripheral blood mononuclear cells by bioactivity-guided fractionation".PHYTOMEDICINE 20.13(2013):1196-1202. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。