A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish
文献类型：期刊论文
| 作者 | Ullah, Najeeb1,2; Noureen, Beenish3; Zahra, Qurat Ul Ain4; Aziz, Tariq5; Shehzadi, Somia6; Alfaifif, Mohammad Y.7; Elbehairif, Serag Eldin I.7; Thebo, Khalid Hussain8; Ullah, Asmat9; Iqbal, Haroon10 |
| 刊名 | CURRENT ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2023 |
| 卷号 | 19期号:9页码:677-684 |
| 关键词 | Aptamer fluorescent sensing saxitoxin mesoporous silica nanoparticles(,) marine toxins shellfish poisoning |
| ISSN号 | 1573-4110 |
| DOI | 10.2174/0115734110269897231020065609 |
| 通讯作者 | Ullah, Asmat(asmat_masood@yahoo.com) ; Iqbal, Haroon(harooniqbal415@hotmail.com) |
| 英文摘要 | Background: Saxitoxin (STX) stands as one of the most potent marine biotoxins, exhibiting high lethality. Despite its severity, current treatments remain ineffective, and existing detection techniques are limited due to ethical concerns and technical constraints.Methods: Herein, an innovative approach was constructed for STX detection, utilizing mesoporous silica nanoparticles (MSN) as a foundation. This innovative, easy, and label-free aptamer (Apt)-sensor was fabricated. Apts were employed as molecular identification probes and "gated molecules," while rhodamine 6G was encapsulated within particles to serve as a signal probe. In a lack of STX, Apts immobilized on an MSN surface kept a "gate" closed, preventing signal probe leakage. Upon the presence of STX, the "gate" opened, allowing a particular binding of Apts to STX and a subsequent release of a signal probe.Results: Experimental results demonstrated a positive correlation between fluorescence intensity and concentrations of STX within a range of 1 to 80 nM, with an exceptional limit of detection of 0.12 nM. Furthermore, the selectivity and stability of a biosensor were rigorously evaluated, validating its reliability.Conclusion: This newly developed sensing strategy exhibits remarkable performance in STX detection. Its success holds significant promise for advancing portable STX detection equipment, thereby addressing a pressing need for efficient and ethical detection methods in combating marine biotoxin contamination. |
| 资助项目 | Deanship of Scientific Research at King Khalid University[R.G.P. 2/ 59 /44] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001134359900003 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| 资助机构 | Deanship of Scientific Research at King Khalid University |
| 源URL |  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Ullah, Asmat; Iqbal, Haroon |
| 作者单位 | 1.Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Appl Surface & Colloid Chem, Xian 710119, Peoples R China 2.Univ Tennessee, Dept Chem Engn, Chattanooga, TN 37403 USA 3.Xi An Jiao Tong Univ, Inst Med Engn, Sch Basic Med Sci, Dept Biophys,Hlth Ctr, Xian 710061, Shaanxi, Peoples R China 4.Univ Sci & Technol China USTC, Biomed Imaging Ctr, Hefei, Peoples R China 5.Jiangsu Univ, Fac Civil Engn & Mech, Zhenjiang 212013, Peoples R China 6.Univ Lahore, Univ Inst Med Lab Technol, Lahore 54000, Pakistan 7.King Khalid Univ, Fac Sci, Dept Biol, Abha 9004, Saudi Arabia 8.Chinese Acad Sci, Inst Met Res, Shenyang, Peoples R China 9.Zhejiang Prov Peoples Hosp, Clin Res Inst, Hangzhou 310014, Zhejiang, Peoples R China 10.Chinese Acad Sci Hangzhou, Zhejiang Canc Hosp, Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ullah, Najeeb,Noureen, Beenish,Zahra, Qurat Ul Ain,et al. A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish[J]. CURRENT ANALYTICAL CHEMISTRY,2023,19(9):677-684. |
| APA | Ullah, Najeeb.,Noureen, Beenish.,Zahra, Qurat Ul Ain.,Aziz, Tariq.,Shehzadi, Somia.,...&Iqbal, Haroon.(2023).A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish.CURRENT ANALYTICAL CHEMISTRY,19(9),677-684. |
| MLA | Ullah, Najeeb,et al."A Novel Fluorescent Aptasensor Based on Mesoporous Silica Nanoparticles for Selective and Sensitive Detection of Saxitoxin in Shellfish".CURRENT ANALYTICAL CHEMISTRY 19.9(2023):677-684. |
入库方式： OAI收割
来源：金属研究所
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