In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy
文献类型：期刊论文
| 作者 | Hu, Jiyuan1,2; Wang, Zhenyu2; Hu, Hao3; Zhao, Jing4; Li, Hongwei2; Zhang, Xinyu2; Bi, Jianbin1; Li, Jianzhong1,2 |
| 刊名 | ACTA BIOMATERIALIA
 |
| 出版日期 | 2023-12-01 |
| 卷号 | 172页码:321-329 |
| 关键词 | Ureteral stricture Bilayer drug-eluting Ureteral stent Rapamycin Lithotripsy |
| ISSN号 | 1742-7061 |
| DOI | 10.1016/j.actbio.2023.10.009 |
| 通讯作者 | Hu, Hao(huhao@bjmu.edu.cn) ; Li, Jianzhong(174030098@qq.com) |
| 英文摘要 | Ureteral stricture caused by holmium: YAG laser lithotripsy is one of the most challenging issues for urologists. Currently, evidence for rapamycin application in reducing ureterostenosis is not sufficient. This study aimed to assess the inhibition of ureteral stricture of rapamycin-eluting stents in vitro and in vivo. A bilayered drug-eluting ureteral stent consisted of drug blending with poly (lactic-co-glycolic acid) (PU/drug stent), which was over-layered by polycaprolactone (PCL) by ultrasonic atomizing spraying. Stent morphology was observed by scanning electron microscope. A kidney-ureter-bladder model was established to simulate the stents-releasing condition, and high-performance liquid chromatography was used to measure the drug release rate. The inhibitory proliferation was detected by CCK-8. The bladder of rats was injured through electro tome, and stents were implanted for 7, 14, and 28 days. The effects of drug-eluting stents was investigated by hematoxylin-eosin staining, immunofluorescence staining, realtime quantitative polymerase chain reaction and western blot. The bilayered stents could block the burst loss of the drug and maintained a sustained delivery period because of the 5.3 mu m thickness of the PCL layer. The relative growth rates of cells plotted inhibitory effect on the proliferation of human urethral scar fibroblast cells. For in vivo results of 28 days, the bilayered stent maintained structural integrity and induced less deposition of crystals, thinner and less lamina propria connective tissues were formed, and alpha-SMA and TGF-beta 1 were downregulated. Bilayered rapamycin-eluting stent is significantly effective in alleviating fibrosis in in vitro and in vivo models. |
| 资助项目 | Shenyang Public Health Science and Technology Project[21-172-9-10] ; Capital's Funds for Health Improvement and Research[2020-2Z-40810] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001154340700001 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | Shenyang Public Health Science and Technology Project ; Capital's Funds for Health Improvement and Research |
| 源URL |  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Hu, Hao; Li, Jianzhong |
| 作者单位 | 1.China Med Univ, Affiliated Hosp 1, Dept Urol, Shenyang 110002, Peoples R China 2.Gen Hosp Northern Theater Command, Dept Urol, Shenyang 110840, Peoples R China 3.Peking Univ, Dept Urol, Peoples Hosp, Beijing 100044, Peoples R China 4.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Jiyuan,Wang, Zhenyu,Hu, Hao,et al. In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy[J]. ACTA BIOMATERIALIA,2023,172:321-329. |
| APA | Hu, Jiyuan.,Wang, Zhenyu.,Hu, Hao.,Zhao, Jing.,Li, Hongwei.,...&Li, Jianzhong.(2023).In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy.ACTA BIOMATERIALIA,172,321-329. |
| MLA | Hu, Jiyuan,et al."In vitro and in vivo assessment of a bilayered degradable rapamycin-eluting stent for ureteral stricture caused by holmium: YAG laser lithotripsy".ACTA BIOMATERIALIA 172(2023):321-329. |
入库方式： OAI收割
来源：金属研究所
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