SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis
文献类型：期刊论文
| 作者 | Pan, Peng; Yu, Xinding; Chen, Tiantian; Liu, Wentao |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2024-09-01 |
| 卷号 | 241页码:12 |
| 关键词 | Hyaline cartilage regeneration Nonphysical barrier SOX9+functionalized scaffolds Cartilage fibrosis |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2024.114011 |
| 通讯作者 | Liu, Wentao(wtliu@imr.ac.cn) |
| 英文摘要 | Hyaline cartilage regeneration will bring evangel to millions of people suffered from cartilage diseases. However, uncontrollable cartilage fibrosis and matrix mineralization are the primary causes of cartilage regeneration failure in many tissue engineering scaffolds. This study presents a new attempt to avoid endochondral ossification or fibrosis in cartilage regeneration therapy by establishing biochemical regulatory area. Here, SOX9 expression plasmids are assembled in cellulose gels by chitosan gene vectors to fabricate SOX9+ functionalized scaffolds. RT-qPCR, western blot and biochemical analysis all show that the SOX9 reinforcement strategy can enhance chondrogenic specific proteins expression and promote GAG production. Notably, the interference from SOX9 has resisted osteogenic inducing significantly, showing an inhibition of COL1, OPN and OC production, and the inhibition efficiency was about 58.4 %, 22.8 % and 76.9 % respectively. In vivo study, implantation of these scaffolds with BMSCs can induce chondrogenic differentiation and resist endochondral ossification effectively. Moreover, specific SOX9+ functionalized area of the gel exhibited the resistance to matrix mineralization, indicating the special biochemical functional area for cartilage regeneration. These results indicate that this strategy is effective for promoting the hyaline cartilage regeneration and avoiding cartilage fibrosis, which provides a new insight to the future development of cartilage regeneration scaffolds. |
| 资助项目 | Applied Basic Research Programs of Liaoning Province[2022JH2/101300104] ; Interdisciplinary Convergence Foundation of Medical Engineering of Natural Science Foundation of Liaoning Province[2022-YGJC-02] ; Shenyang National Laboratory for Materials Science ; Institute of Metal Research, Chinese Academy of Sciences[E01SL607] ; Joint Open Fund of State Key Laboratory of Robotics[2022-KF-12-01] ; China Medical University (Shenyang, China) |
| WOS研究方向 | Biophysics ; Chemistry ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001250588400001 |
| 出版者 | ELSEVIER |
| 资助机构 | Applied Basic Research Programs of Liaoning Province ; Interdisciplinary Convergence Foundation of Medical Engineering of Natural Science Foundation of Liaoning Province ; Shenyang National Laboratory for Materials Science ; Institute of Metal Research, Chinese Academy of Sciences ; Joint Open Fund of State Key Laboratory of Robotics ; China Medical University (Shenyang, China) |
| 源URL |  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Liu, Wentao |
| 作者单位 | Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Peng,Yu, Xinding,Chen, Tiantian,et al. SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2024,241:12. |
| APA | Pan, Peng,Yu, Xinding,Chen, Tiantian,&Liu, Wentao.(2024).SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis.COLLOIDS AND SURFACES B-BIOINTERFACES,241,12. |
| MLA | Pan, Peng,et al."SOX9 functionalized scaffolds as a barrier to against cartilage fibrosis".COLLOIDS AND SURFACES B-BIOINTERFACES 241(2024):12. |
入库方式： OAI收割
来源：金属研究所
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