Vertically Stacked Paper-Based Microarray Device for High-Throughput SERS Detection of Two Cancer Biomarkers
文献类型：期刊论文
| 作者 | Qi, Ji3; Chen, Jiadong3; Yu, Qian3; Lu, Mengdan3; Joung, Younju3; Yu, Xiangdong3; Lee, Mi-Kyung1; Chen, Lingxin2; Choo, Jaebum3 |
| 刊名 | SMALL
 |
| 出版日期 | 2026-03-31 |
| 页码 | 12 |
| 关键词 | high-throughput biomarker detection immunoassay microfluidic paper chip rapid detection surface enhanced Raman scattering analysis |
| ISSN号 | 1613-6810 |
| DOI | 10.1002/smll.202514850 |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr) |
| 英文摘要 | The demand for point-of-care (POC) diagnostics is driving portable analytical innovation. Despite advances, precise optical tag control remains a key challenge limiting diagnostic accuracy. Here, we present a vertically stacked microarray paper-based device (& micro;APAD) enabling high-throughput surface-enhanced Raman scattering (SERS) immunoassays for multiplex biomarker detection. The 16-layer wax-patterned architecture integrates sample migration, reaction, and capture within one platform. Optimized microfluidic channels ensure uniform nanotag distribution, reducing signal variation from 36.6% to 6.69%, while an agarose hydrogel layer regulates flow to improve immunocapture efficiency. The & micro;APAD simultaneously analyzes 14 samples for two biomarkers within 30 min. Using carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as models, it achieves detection limits of 0.34 and 0.69 ng/mL, outperforming conventional ELISA. Tests with spiked serum confirm high analytical accuracy, showing recoveries of 80.3%-139% and RSDs of 6.30%-10.8%. With its high sensitivity, flow precision, scalability, and true multiplexing capability, this low-cost platform offers great potential for next-generation POC diagnostics and large-scale health monitoring. |
| WOS关键词 | PERMEABILITY ; ASSAYS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:001728810700001 |
| 资助机构 | Chinese Academy of Sciences ; Ministry of Trade, Industry, and Energy ; Regional Innovation System Education ; Key Deployment Project of Centre for Ocean Mega-Research of Science ; National Natural Science Foundation of China ; National Research Foundation of Korea ; Seoul RISE Center |
| 源URL | [http://ir.yic.ac.cn/handle/133337/42329]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Chen, Lingxin; Choo, Jaebum |
| 作者单位 | 1.Chung Ang Univ, Coll Med, Dept Lab Med, Seoul, South Korea 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 3.Chung Ang Univ, Dept Chem, Seoul, South Korea |
| 推荐引用方式 GB/T 7714 | Qi, Ji,Chen, Jiadong,Yu, Qian,et al. Vertically Stacked Paper-Based Microarray Device for High-Throughput SERS Detection of Two Cancer Biomarkers[J]. SMALL,2026:12. |
| APA | Qi, Ji.,Chen, Jiadong.,Yu, Qian.,Lu, Mengdan.,Joung, Younju.,...&Choo, Jaebum.(2026).Vertically Stacked Paper-Based Microarray Device for High-Throughput SERS Detection of Two Cancer Biomarkers.SMALL,12. |
| MLA | Qi, Ji,et al."Vertically Stacked Paper-Based Microarray Device for High-Throughput SERS Detection of Two Cancer Biomarkers".SMALL (2026):12. |
入库方式： OAI收割
来源：烟台海岸带研究所
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