Graphene oxide-integrated double-stranded DNA mismatch regulation on a microfluidic paper-based device for ultrasensitive fluorescent detection of Vibrio parahaemolyticus and Shigella flexneri
文献类型：期刊论文
| 作者 | Sun, Hongxiao3,4; Tan, Jiangkun3,4; Wu, Yixuan4; Wang, Siyu1; Li, Bowei3,4; Wang, Xiaoyan2; Wang, Yunqing4; Zhang, Xin1; Chen, Lingxin1,3,4 |
| 刊名 | MICROCHEMICAL JOURNAL
 |
| 出版日期 | 2026-06-01 |
| 卷号 | 225页码:9 |
| 关键词 | Microfluidic paper-based sensor Vibrio parahaemolyticus Shigella flexneri Graphene oxide Aptamer |
| ISSN号 | 0026-265X |
| DOI | 10.1016/j.microc.2026.117950 |
| 通讯作者 | Li, Bowei(bwli@yic.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn) |
| 英文摘要 | The escalating burden of foodborne pathogens-particularly Vibrio parahaemolyticus (Vp) and Shigella flexneri (Sf)-poses a pressing challenge to public health and food safety. Conventional detection assays can no longer meet the demands of modern surveillance, highlighting the urgent need for detection methods with the combined advantages of high efficiency, sensitivity, and simplicity. Herein, we report a graphene oxide (GO)-integrated microfluidic paper-based sensor for the ultrasensitive detection of these two pathogens, which employs a double-stranded DNA (dsDNA) probe with tunable base mismatches. Dual-pathogen testing is implemented on a single device through two independent, spatially separated detection zones, each designed for one target bacterium. The dsDNA probe is immobilized on the paper substrate through the specific streptavidin-biotin interaction, where a FAM-labelled complementary DNA (cDNA) strand acts as the fluorescent reporter. In the absence of target pathogens, the dsDNA duplex remains stable, yielding a strong fluorescent signal. Upon exposure to target pathogens, specific aptamer-target binding induces dsDNA duplex dissociation. The released cDNA is then adsorbed onto GO via pi-pi stacking interactions, and the subsequent Fo & uml;rster resonance energy transfer (FRET) between GO and the FAM label quenches the fluorescence, resulting in a "signal-off" readout. This sensor exhibits a broad linear dynamic range (101-109 CFU mL-1), low limits of detection (LODs: 5.5 CFU mL-1 for Vp and 4.2 CFU mL-1 for Sf), excellent specificity, and high stability. In real sample analyses, it achieves satisfactory recovery rates (86.60-109.28%). This work presents an efficient, reliable, and easy-to-operate fluorescent paper-based platform for the rapid detection of foodborne pathogens, holding great promise for on-site surveillance in food safety and public health. |
| WOS关键词 | HIGHLY SENSITIVE DETECTION ; RAPID DETECTION ; FOOD ; APTAMER ; APTASENSOR ; PLATFORM ; PROTEIN ; PROBE |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001741107400001 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/42372]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Li, Bowei; Zhang, Xin; Chen, Lingxin |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, LaoShan Lab, Qingdao 266237, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Coastal Zone Ecol Environm Monitoring Technol & Eq, Shandong Key Lab Coastal Zone Environm Proc & Ecol, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Hongxiao,Tan, Jiangkun,Wu, Yixuan,et al. Graphene oxide-integrated double-stranded DNA mismatch regulation on a microfluidic paper-based device for ultrasensitive fluorescent detection of Vibrio parahaemolyticus and Shigella flexneri[J]. MICROCHEMICAL JOURNAL,2026,225:9. |
| APA | Sun, Hongxiao.,Tan, Jiangkun.,Wu, Yixuan.,Wang, Siyu.,Li, Bowei.,...&Chen, Lingxin.(2026).Graphene oxide-integrated double-stranded DNA mismatch regulation on a microfluidic paper-based device for ultrasensitive fluorescent detection of Vibrio parahaemolyticus and Shigella flexneri.MICROCHEMICAL JOURNAL,225,9. |
| MLA | Sun, Hongxiao,et al."Graphene oxide-integrated double-stranded DNA mismatch regulation on a microfluidic paper-based device for ultrasensitive fluorescent detection of Vibrio parahaemolyticus and Shigella flexneri".MICROCHEMICAL JOURNAL 225(2026):9. |
入库方式： OAI收割
来源：烟台海岸带研究所
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