Key metabolites secreted by Chlorella vulgaris alleviate salt stress in soybean seedlings
文献类型：期刊论文
| 作者 | Shi, Yunyi3,4; Chen, Ruitong4; Jiang, Kai4; Jiang, Aijuan4; Yang, Jianchao2; Cui, Hongli1; Chen, Min3,4 |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2026-03-04 |
| 页码 | 19 |
| 关键词 | Chlorella exosomes inosine linolenic acid salt stress soybean |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.70207 |
| 通讯作者 | Cui, Hongli(hlcui@yic.ac.cn) ; Chen, Min(chenminrundong@sdnu.edu.cn) |
| 英文摘要 | Soil salinization is a major abiotic stress factor that reduces soybean production. Studies have shown that application of Chlorella promotes plant growth; however, its potential to mitigate salt stress, and the active components involved and the underlying mechanisms, remains unclear. In this study, the application of Chlorella vulgaris significantly mitigated salt stress in soybean seedlings. To identify the active components, we fractionated C. vulgaris and found that the extracellular secretions were primarily responsible for the mitigation. Furthermore, we isolated and characterized exosomes from these secretions. Phenotypic and physiological assessments confirmed that C. vulgaris exosomes alleviated salt stress in soybean seedlings to an extent similar to the intact organism. We identified the key metabolites (linolenic acid and inosine) as the active components within these exosomes. Notably, when applied in combination, they showed a strong synergistic effect, collectively promoting seedling growth, restoring ion homeostasis, and improving redox homeostasis under salt stress. To investigate the molecular mechanisms of salt stress alleviation, the transcriptomes of soybean seedlings subjected to different treatments were analyzed. Salt stress induced widespread changes in gene expression; however, application of linoleic acid (LA), inosine, and especially their combination, led to substantial transcriptomic reprogramming. Enrichment analysis indicated that these modifications were coordinated across energy metabolism, redox homeostasis, carbohydrate metabolism, and ion transport. Importantly, the LA + inosine combination induced a synergistic rather than simply additive transcriptional response, which explains its superior effect on salt tolerance at the molecular level. In this study, we successfully isolated and identified, for the first time, exosomes as the functional components through which C. vulgaris alleviates salt stress in soybean seedlings. Further analysis identified LA and inosine within the exosomes as the key active metabolites. These findings provide a novel theoretical basis for improving crop salt tolerance and are important for developing novel algal nanovesicle-based biostimulants. |
| WOS关键词 | MALONDIALDEHYDE MDA ; OXIDATIVE STRESS ; MECHANISMS ; TOLERANCE ; SALINITY ; NA+ ; K+ |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001706801900001 |
| 资助机构 | Joint Funds of the National Natural Science Foundation of China ; Shandong Province ; Shandong Province Key R&D Plan (Rural Revitalization Science and Technology Innovation Boosting Action Plan) Project ; Shandong Provincial Natural Science Research Foundation Project ; China Agriculture Research System ; Dongying Comprehensive Experimental Station of Shandong Province Forage Industry Technology System |
| 源URL | [http://ir.yic.ac.cn/handle/133337/42222]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 |
| 通讯作者 | Cui, Hongli; Chen, Min |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 2.Shandong City Serv Inst, Yantai 264003, Peoples R China 3.Natl Ctr Technol Innovat Comprehens Utilizat Salin, Agr Hightech Ind Demonstrat Area Yellow River Delt, Dongying 257000, Peoples R China 4.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Plant Stress Biol & Genet Im, Jinan 250014, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yunyi,Chen, Ruitong,Jiang, Kai,et al. Key metabolites secreted by Chlorella vulgaris alleviate salt stress in soybean seedlings[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2026:19. |
| APA | Shi, Yunyi.,Chen, Ruitong.,Jiang, Kai.,Jiang, Aijuan.,Yang, Jianchao.,...&Chen, Min.(2026).Key metabolites secreted by Chlorella vulgaris alleviate salt stress in soybean seedlings.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,19. |
| MLA | Shi, Yunyi,et al."Key metabolites secreted by Chlorella vulgaris alleviate salt stress in soybean seedlings".JOURNAL OF INTEGRATIVE PLANT BIOLOGY (2026):19. |
入库方式： OAI收割
来源：烟台海岸带研究所
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