Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry
文献类型：期刊论文
| 作者 | Ye, Yifei2,3; Geng, Xiaokun4; Kong, Qi4; Li, Xue1; Zhi, Tian5; Zhang, Lingqian2; Li, Mingxiao2; Huang, Chengjun2; Zhao, Yang2; Zhao, Haiping1 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2026-02-15 |
| 卷号 | 449页码:11 |
| 关键词 | Specific membrane capacitance Microfluidic impedance cytometry Macrophage polarization Drug screening Stroke |
| DOI | 10.1016/j.snb.2025.139191 |
| 英文摘要 | Macrophages are highly plastic immune cells that adopt diverse functional states to modulate inflammation. Capturing this dynamic heterogeneity requires single-cell resolution. However, conventional fluorescence flow cytometry relies on multistep antibody labeling and lacks a direct quantitative read-out of cell-intrinsic properties. In this study, we identify specific membrane capacitance (Csm), an intrinsic electrical property of individual cells, as a sensitive and quantitative marker of macrophage polarization. Measured at single-cell resolution using high-throughput, label-free microfluidic impedance cytometry, Csm increases in pro-inflammatory states and decreases in anti-inflammatory conditions, offering a direct readout of functional macrophage states. Leveraging this metric, we establish a rapid drug screening platform targeting macrophage polarization. Notably, screening the histone deacetylase (HDAC) family reveals that the inhibition of Class IIa HDACs or the activation of Class III HDACs effectively suppresses pro-inflammatory polarization. These effects are validated using blood samples from stroke patients and further supported by improvement of ischemic outcomes in mouse cerebral ischemic models. Collectively, our results highlight Csm as a robust, label-free marker for real-time single-cell analysis of macrophage polarization, offering a valuable tool for both basic research and therapeutic development in neuroinflammation. |
| 资助项目 | National Natural Science Foundation of China[62474193] ; National Natural Science Foundation of China[62171441] ; National Natural Science Foundation of China[82271309] ; Beijing Hospitals Authority Clinical Medicine Development[YGLX202328] ; Key Research Special Project of Beijing Natural Science Foundation[Z240021] ; Opening Project of Key Laboratory of Fabrication Technologies for Integrated Circuits, Chinese Academy of Sciences |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:001629750600004 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/42969]  |
| 专题 | 中国科学院计算技术研究所 |
| 通讯作者 | Zhao, Yang; Zhao, Haiping |
| 作者单位 | 1.Capital Med Univ, Xuanwu Hosp, Inst Cerebrovascular Dis Res, Beijing 100053, Peoples R China 2.Chinese Acad Sci, Inst Microelect, Beijing 100029, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, 2020 X Lab, Shanghai 200050, Peoples R China 4.Capital Med Univ, Beijing Luhe Hosp, Dept Neurol, Beijing 101149, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 101125, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Yifei,Geng, Xiaokun,Kong, Qi,et al. Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry[J]. SENSORS AND ACTUATORS B-CHEMICAL,2026,449:11. |
| APA | Ye, Yifei.,Geng, Xiaokun.,Kong, Qi.,Li, Xue.,Zhi, Tian.,...&Zhao, Haiping.(2026).Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry.SENSORS AND ACTUATORS B-CHEMICAL,449,11. |
| MLA | Ye, Yifei,et al."Label-free detection of macrophage polarization via specific membrane capacitance using high-throughput microfluidic impedance cytometry".SENSORS AND ACTUATORS B-CHEMICAL 449(2026):11. |
入库方式： OAI收割
来源：计算技术研究所
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