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A universal and cost-efficient sample labeling approach for multiplexed single-cell RNA-seq based on recombinant HUH-endonuclease-agglutinin tagging

文献类型：期刊论文

作者Zhang, Quanyong6,7; Li, Maorong6,7; Shen, Luemou6,7; Chen, Lujia6; Yuan, Zhiheng9; Zhang, Xu2; Wang, Hao3; Yu, Yang9; Luo, Fucheng6; Peng, Guangdun4
刊名JOURNAL OF GENETICS AND GENOMICS
出版日期2026-04-01
卷号53期号:4页码:744-757
关键词Single-cell RNA sequencing Sample labeling HUH-endonuclease Agglutinin Glycan
ISSN号1673-8527
DOI10.1016/j.jgg.2025.10.004
通讯作者Song, Ning(ningsong@szu.edu.cn) ; Chen, Kai(chen_kai@gmlab.ac.cn)
英文摘要Recent advances in single-cell transcriptomics have revolutionized our understanding of cellular diversity and tissue heterogeneity, providing unprecedented insights into biological and medical research. However, the high per-assay cost limits broader applications of this technology. Although sample labeling strategies enabling multiplexing have emerged, current methods suffer from either impractical complexity for barcoding or high cost for preparing the index labeling reagents. To address these challenges, here we present HUH-endonuclease-agglutinin tagging (HEATag), a universal cell membrane labeling approach that combines Duck circovirus HUH endonuclease (DCV) with wheat germ agglutinin (WGA) to efficiently tag cell membranes with indexed single-stranded DNA(ssDNA). The DCV domain enables rapid, sequence-specific conjugation of indexed ssDNA, while the WGA domain ensures robust labeling of fresh or fixed cells across diverse species. This method is compatible with both commercial platforms and custom systems, readily adaptable to various single-cell omics workflows. Therefore, HEATag provides a universal, cost-effective, and scalable solution for high-throughput single-cell studies, enhancing library preparation efficiency and minimizing batch effects for single-cell researchers. Copyright (c) 2025, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
WOS关键词PROTEIN ; DNA ; ATLAS
资助项目National Key Research and Development Program of China[2023YFC3402700] ; Yunnan Talents Program[XDYC-PYJL-2022-0020] ; Principal Investigator Project of the Southern Marine Science and Engineering Guangdong Laboratory[YQ2024004] ; Natural Science Foundation of Yunnan Province[202102AA100053] ; Guangzhou National Laboratory[GZNL2023A03005] ; National Thousand Talents Program[XDYC-PYJL-2022-0020]
WOS研究方向Biochemistry & Molecular Biology ; Genetics & Heredity
语种英语
WOS记录号WOS:001737874600001
出版者SCIENCE PRESS
源URL[http://ir.qdio.ac.cn/handle/337002/204934]  
专题中国科学院海洋研究所
通讯作者Song, Ning; Chen, Kai
作者单位1.Ocean Univ China, Key Lab Trop Aquat Germplasm Hainan Prov, Qingdao 266100, Shandong, Peoples R China
2.Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cell & Dev Biol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266000, Shandong, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China
5.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao 266100, Shandong, Peoples R China
6.Kunming Univ Sci & Technol, Inst Primate Translat Med, State Key Lab Primate Biomed Res, Kunming 650500, Yunnan, Peoples R China
7.Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou 511458, Guangdong, Peoples R China
8.Shenzhen Univ, Inst Adv Study, Shenzhen Key Lab Marine Microbiome Engn,Key Lab, Archaeal Biol Ctr,Synthet Biol Res Ctr, Shenzhen 518060, Peoples R China
9.Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, GMU GIBH Joint Sch Life Sci, Guangdong Hong Kong Macao Joint Lab Cell Fate Regu, Guangzhou 510623, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Quanyong,Li, Maorong,Shen, Luemou,et al. A universal and cost-efficient sample labeling approach for multiplexed single-cell RNA-seq based on recombinant HUH-endonuclease-agglutinin tagging[J]. JOURNAL OF GENETICS AND GENOMICS,2026,53(4):744-757.
APA Zhang, Quanyong.,Li, Maorong.,Shen, Luemou.,Chen, Lujia.,Yuan, Zhiheng.,...&Chen, Kai.(2026).A universal and cost-efficient sample labeling approach for multiplexed single-cell RNA-seq based on recombinant HUH-endonuclease-agglutinin tagging.JOURNAL OF GENETICS AND GENOMICS,53(4),744-757.
MLA Zhang, Quanyong,et al."A universal and cost-efficient sample labeling approach for multiplexed single-cell RNA-seq based on recombinant HUH-endonuclease-agglutinin tagging".JOURNAL OF GENETICS AND GENOMICS 53.4(2026):744-757.

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来源：海洋研究所

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