A Transcription Factor-based Framework for Monitoring Stress Status in Oysters: Applications in Conservation and Restoration
文献类型：期刊论文
| 作者 | Wang, Shen-Tong2,3,6; Li, Li2,3,4,5,6; Yang, Qi2; Wang, Wei2,3,6; Liu, Ming-Kun1,2,5,6; Wang, Xin-Xing2; Wang, Yu7; Zhang, Guo-Fan1,2,4,5,6 |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2025-11-18 |
| 卷号 | 27期号:6页码:18 |
| 关键词 | Health status Molecular indicators Oyster reefs Tidal stress Transcription factors |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-025-10539-5 |
| 通讯作者 | Li, Li(lili@qdio.ac.cn) |
| 英文摘要 | Developing effective molecular indicators to monitor stress status of coastal species is a top priority due to the impacts of climate change. However, the complexity of stress responses, which are regulated by multiple genes, limits the effectiveness of single-gene approaches in accurately reflecting stress status. Transcription factors (TFs) are promising candidates for comprehensively assessing stress responses, as they regulate numerous stress-responsive genes. In this study, we present a framework for identifying TF indicators that reflect the stress status of Pacific oysters (Crassostrea gigas). Specifically, oysters were exposed to high, medium, and low tide conditions to assess the physiological responses of oysters to tidal-induced stress. Enrichment analysis of differentially accessible chromatin peaks derived from assay for transposase-accessible chromatin sequencing (ATAC-seq) identified several key TFs. Among these, CCCTC-binding factor (Cg-CTCF) and MYB proto-oncogene A (Cg-MYBA) were significantly upregulated under tidal-induced stress and occupied critical positions in regulatory networks, as indicated by RNA-seq. RNA interference experiments confirmed that both genes contribute to enhancing survival under heat stress, a major stressor affecting oysters. Additionally, field experiments demonstrated significant upregulation of these genes under natural stress conditions, suggesting their potential as indicators for oyster reef management. To our knowledge, this is the first study on the coastal invertebrate to combine ATAC/RNA-seq with in vivo TF knockdown and field validation to propose TFs as practical stress indicators. We advocate for the broad application of our framework to explore TFs as molecular indicators of health status in marine organisms, thereby enabling informed strategies for conservation management. |
| WOS关键词 | PACIFIC OYSTER ; SALT STRESS ; MYTILUS-CALIFORNIANUS ; CRASSOSTREA-GIGAS ; CTCF ; GALLOPROVINCIALIS ; ADAPTATION ; COMPLEXITY ; HEMOCYTES ; MORTALITY |
| 资助项目 | China Agriculture Research System of MOF and MARA[CARS-49] ; National Natural Science Foundation of China[42406096] ; National Natural Science Foundation of China[42306108] ; Key Research and Development Program of Shandong[ZFJH202309] ; National Key Research and Development Program of China[2023YFD2401102] ; Department of Science & Technology of Shandong Province[2022GJJLJRC02-096] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001618370000002 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204324]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Li |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266000, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China 5.Shandong Ctr Technol Innovat Oyster Seed Ind, Qingdao 266105, Peoples R China 6.Natl & Local Joint Engn Lab Ecol Mariculture, Inst Oceanol, Qingdao 266071, Peoples R China 7.Tianjin Fisheries Res Inst, Tianjin 300221, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shen-Tong,Li, Li,Yang, Qi,et al. A Transcription Factor-based Framework for Monitoring Stress Status in Oysters: Applications in Conservation and Restoration[J]. MARINE BIOTECHNOLOGY,2025,27(6):18. |
| APA | Wang, Shen-Tong.,Li, Li.,Yang, Qi.,Wang, Wei.,Liu, Ming-Kun.,...&Zhang, Guo-Fan.(2025).A Transcription Factor-based Framework for Monitoring Stress Status in Oysters: Applications in Conservation and Restoration.MARINE BIOTECHNOLOGY,27(6),18. |
| MLA | Wang, Shen-Tong,et al."A Transcription Factor-based Framework for Monitoring Stress Status in Oysters: Applications in Conservation and Restoration".MARINE BIOTECHNOLOGY 27.6(2025):18. |
入库方式： OAI收割
来源：海洋研究所
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