Functional characterization of an MPEG1 homologue from Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Chen, Yuan3; Zhang, Jian3; Li, Xuepeng3; Sun, Boguang1,2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2026-02-01 |
| 卷号 | 169页码:8 |
| 关键词 | Macrophage-expressed gene 1 Paralichthys olivaceus Vibrio harveyi Edwardsiella piscicida |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2025.111039 |
| 通讯作者 | Sun, Boguang(sunboguang@qdio.ac.cn) |
| 英文摘要 | MPEG1 (macrophage-expressed gene 1) is a membrane attack complex/perforin (MACPF) superfamily protein implicated in antimicrobial defense, yet its functional mechanisms in teleost fish remain poorly understood. In this study, we identified and characterized an MPEG1 homologue (PoMPEG1) from Paralichthys olivaceus (Japanese flounder). PoMPEG1 encodes a 710-amino-acid protein comprising a conserved MACPF-P2 ectodomain, a transmembrane helix, and a short cytosolic tail, and localizes to lysosomal compartments. PoMPEG1 was ubiquitously expressed in flounder tissues and strongly induced upon bacterial infection. The cytosolic tail of PoMPEG1 contains a cationic segment with potent bactericidal activity against Vibrio harveyi. Structural and functional analyses revealed that this activity depends on an intact alpha-helical conformation and is inhibited by an adjacent anionic segment, suggesting a regulatory mechanism controlling its activation. In contrast, the ectodomain of PoMPEG1 likely mediated defense against the intracellular pathogen Edwardsiella piscicida. This is supported by the observation that PoMPEG1 knockdown increased the intracellular bacterial load, whereas the introduction of the intact protein, but not its ectodomain-deficient mutants, reduced it. Together, these findings demonstrate that PoMPEG1 functions as a dual-action immune effector employing distinct mechanisms to combat extracellular and intracellular pathogens, providing new insights into MPEG1-mediated host defense in teleost fish. |
| WOS关键词 | PERFORIN-2 ; EXPRESSION ; IDENTIFICATION ; ABALONE ; PROTEIN |
| 资助项目 | Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001630887600001 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204351]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Boguang |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266000, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Yantai Univ, Sch Ocean, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yuan,Zhang, Jian,Li, Xuepeng,et al. Functional characterization of an MPEG1 homologue from Paralichthys olivaceus[J]. FISH & SHELLFISH IMMUNOLOGY,2026,169:8. |
| APA | Chen, Yuan,Zhang, Jian,Li, Xuepeng,&Sun, Boguang.(2026).Functional characterization of an MPEG1 homologue from Paralichthys olivaceus.FISH & SHELLFISH IMMUNOLOGY,169,8. |
| MLA | Chen, Yuan,et al."Functional characterization of an MPEG1 homologue from Paralichthys olivaceus".FISH & SHELLFISH IMMUNOLOGY 169(2026):8. |
入库方式： OAI收割
来源：海洋研究所
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